Nucleotide sequences in Xenopus 5S DNA required for transcription termination

  title={Nucleotide sequences in Xenopus 5S DNA required for transcription termination},
  author={Daniel F. Bogenhagen and Donald D. Brown},

Analysis of the signals for transcription termination by purified RNA polymerase II.

It is suggested that a structural element causing a bend in the DNA helix may be part of the signal for transcription termination by purified RNA polymerase II.

Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.

It is concluded that the promoter lies somewhere within a region between -320 nucleotide upstream and +113 nucleotides downstream from the site of transcription initiation, which suggests that the duplicated initiation region sequences located further out in the spacer are not required for the normal high densities of RNA polymerase loading seen on ribosomal genes.

Intrinsic sites of transcription termination and pausing in the c-myc gene

The intrinsic sites of transcription termination and pausing described here correspond closely to the 3' ends of transcripts synthesized in Xenopus oocytes injected with plasmids containing the c-myc termination region, suggesting that the intrinsic recognition of these termination and pause sites by purified RNA polymerase II may play a role in the transcription elongation block observed in vivo.

Termination sequence requirements vary among genes transcribed by RNA polymerase III.

It is concluded that pol III termination signals are more complex than hitherto recognized, and that sequence context requirements differ between members of the class 1 and class 2 families of pol III genes.

A tridecamer DNA sequence supports human mitochondrial RNA 3'-end formation in vitro

It is demonstrated that the tridecamer sequence 5'-TGGCAGAGCCCCCCGG-3', contained entirely within the gene for leucyl-tRNA, is necessary to direct accurate termination.

3'-end formation of mouse pre-rRNA involves both transcription termination and a specific processing reaction.

It is shown that termination of mouse ribosomal gene transcription by RNA polymerase I (pol I) takes place in front of an 18-bp DNA sequence element (the 'Sal box'), which was previously shown to function as termination signal.



The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.

The Drosophila tRNAArg gene does not contain an intervening sequence nor the C-C-A sequence corresponding to the 3' terminus of the mature tRNA, which differs only in four positions from that of mouse t RNAArg.

Starting and Stopping Sequences for the RNA Polymerase

This work has sequenced five RNA polymerase-protected DNA fragments that have been sequenced to date and identified those bases that make the critical contacts responsible for maintaining the polymer enzyme-DNA interaction.

Sequence and heterogeneity in the 5S RNA gene cluster of Drosophila melanogaster.

The sequence of the entire 5S RNA gene of Drosophila melanogaster was determined by sequencing collectively 23 copies contained in a cloned fragment of Drosophila DNA and by sequencing individually