Nucleotide sequences in Xenopus 5S DNA required for transcription termination

@article{Bogenhagen1981NucleotideSI,
  title={Nucleotide sequences in Xenopus 5S DNA required for transcription termination},
  author={Daniel F. Bogenhagen and DONALD D. Brown},
  journal={Cell},
  year={1981},
  volume={24},
  pages={261-270}
}
We have analyzed the DNA sequences required for termination of Xenopus 5S RNA synthesis in vitro. Termination occurs within clusters of four or more consecutive T residues in the noncoding DNA strand sequence. The 3' flanking sequence following the gene is not required for termination. The distance between the T cluster and the site of transcription initiation as well as the exact nucleotide sequences preceding the T cluster can be varied without impairing termination. Thus sequence signals for… Expand
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TLDR
It is suggested that sequences important for the termination of RNA synthesis are likely to be conserved between the two Xenopus species and may include the two 3′ terminal nucleotides of the gene itself and the first adjacent two nucleotide in the spacer. Expand
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TLDR
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TLDR
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TLDR
A factor necessary for the accurate transcription of cloned Xenopus 5S genes in vitro has been isolated from soluble extracts of X. laevis ovaries and the site of action has been investigated using the "footprinting" method of Galas and Schmitz (1978). Expand
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