Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.

@article{Zalacan1986NucleotideSO,
  title={Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus.},
  author={Magdalena Zalaca{\'i}n and Antonio Gonz{\'a}lez and M del C Guerrero and Robert J. Mattaliano and Francisco Malpartida and Antonio Jim{\'e}nez},
  journal={Nucleic acids research},
  year={1986},
  volume={14 4},
  pages={1565-81}
}
The nucleotide sequence of a 1467 bp fragment of Streptomyces hygroscopicus DNA containing the gene (hyg) encoding a hygromycin B phosphotransferase (HPH) has been determined. The N-terminal amino acid sequence of HPH determined by automated Edman degradation has allowed the coding sequence of the hyg gene to be identified. The translation initiation triplet is GTG and 5 bp preceding it there is a sequence complementary to the 3'-end of 16S rRNA from S. lividans. The transcriptional start and… CONTINUE READING