Nucleotide sequence of the carA gene and regulation of the carAB operon in Salmonella typhimurium.

@article{Kilstrup1988NucleotideSO,
  title={Nucleotide sequence of the carA gene and regulation of the carAB operon in Salmonella typhimurium.},
  author={Mogens Kilstrup and Chung-Dar Lu and A. Abdelal and Jan Neuhard},
  journal={European journal of biochemistry},
  year={1988},
  volume={176 2},
  pages={421-9}
}
The carAB operon of Salmonella typhimurium encoding carbamoyl-phosphate synthetase (CPSase) has been cloned, and the nucleotide sequence of the first gene of the operon, carA, together with 760 base pairs of the 5'-flanking region was determined. The product of the carA gene is the small subunit of CPSase. It catalyzes the transfer of the amide group from glutamine to an NH3-site on the heavy subunit. Primer extension and S1 nuclease mapping of in vivo carAB transcripts revealed that… CONTINUE READING