Nucleotide sequence of the bean leafroll luteovirus coat protein gene.

@article{Prill1990NucleotideSO,
  title={Nucleotide sequence of the bean leafroll luteovirus coat protein gene.},
  author={Birgit Prill and Edgar Maiss and Lina Katul and Rudolf Casper},
  journal={Nucleic acids research},
  year={1990},
  volume={18 18},
  pages={5544}
}
Purified BLRV RNA was converted to cDNA and cloned into pT7T3 19U phagemid. The nucleotide sequence of a putative bean leafroll luteovirus coat protein gene was determined from the clones pBL9, pBL29 and pBL32 by the chain termination method (1). Sequence analysis reveals a 588 bp open reading frame and an internal 426 bp ORF (Fig. 1). The large ORF is equivalent to a protein of Mr 21.98 kDa, the smaller one encodes a protein of Mr 16.09 kDa. Comparison of the amino acid sequence of the 22 kDa… CONTINUE READING

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