Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome.

@article{Natsuaki1991NucleotideSO,
  title={Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome.},
  author={Tomohide Natsuaki and Mike Mayo and Carol Jolly and A. F. Murant},
  journal={The Journal of general virology},
  year={1991},
  volume={72 ( Pt 9)},
  pages={2183-9}
}
Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5' ORF) and 822 (3' ORF) bases. The 3' ORF is virtually identical in sequence to RNA-3, which encodes the Mr 30509 (30K) coat protein. The 5' ORF encodes an Mr 38860 (39K) protein which slightly resembles the 32K protein encoded by RNA-3 of… CONTINUE READING