# Nucleotide sequence of bacteriophage φX174 DNA

@article{Sanger1977NucleotideSO,
title={Nucleotide sequence of bacteriophage $\phi$X174 DNA},
author={Frederick Sanger and Gillian M. Air and Bart Barrell and Nigel L. Brown and A. R. Coulson and John C. Fiddes and Clyde A. Hutchison and Patrick M. Slocombe and M. Smith},
journal={Nature},
year={1977},
volume={265},
pages={687-695}
}
• Published 24 February 1977
• Biology
• Nature
A DNA sequence for the genome of bacteriophage φX174 of approximately 5,375 nucleotides has been determined using the rapid and simple ‘plus and minus’ method. The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs. Two pairs of genes are coded by the same region of DNA using different reading frames.
1,825 Citations

### Nucleotide sequence of the origin of replication in bacteriophage ΦX174 RF DNA

• Biology, Chemistry
Nature
• 1978
The gene A protein of bacteriophageΦX174 has been used in vitro to convert ΦX RFI DNA into the relaxed RFII form by nicking the viral strand and the nucleotide sequence at the 3′ end of the nick gives the exact position of the origin of Φx RF DNA replication.

### Recognition sequence of bacteriophage ΦX174 gene A protein — an initiator of DNA replication

• Biology, Chemistry
Nature
• 1980
Gene A protein, the initiator protein of bacteriophage ΦX174 DNA replication, cleaves synthetic single-stranded oligodeoxyribonucleotides at the same site as the corresponding sequence at the ΦX

### Potential for variability through multiple gene products of bacteriophage ΦX174

• Biology
Nature
• 1978
The small single-stranded DNA phages ΦX174 and S13 produce multiple products of certain phage genes, as observed by electrophoresis on SDS–polyacrylamide slab gels. Two A protein products, two A*

### Physical structure of the replication origin of bacteriophage lambda.

• Biology
Science
• 1977
The nucleotide sequence of part of the replication region of wild-type bacteriophage lambda and of four mutants defective in the origin of DNA replication (ori-) has been determined and contains a number of unusual features.

### DNA sequence of a region of the φX174 genome coding for a ribosome binding site

• Biology, Chemistry
Nature
• 1977
The DNA region corresponding to a newly identified ribosome binding site in φX174 DNA is sequenced and mapped in relation to the physical map of φX174. Assignments of the binding site to a specific

## References

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### Nucleotide sequence of the origin of replication in bacteriophage ΦX174 RF DNA

• Biology, Chemistry
Nature
• 1978
The gene A protein of bacteriophageΦX174 has been used in vitro to convert ΦX RFI DNA into the relaxed RFII form by nicking the viral strand and the nucleotide sequence at the 3′ end of the nick gives the exact position of the origin of Φx RF DNA replication.

### Isolation and Function of the Gene A Initiator of Bacteriophage ϕX 174, A Highly Specific DNA Endonuclease

• Biology
• 1974
The gene A protein is shown to be a highly specific DNA endonuclease which breaks one phosphodiester bond in the viral strand of either double-stranded, circular ϕX replicative form I (RFI), or in single-stranding, circularπX viral DNA; it is inactive against all other species of DNA tested.

### DNA sequence at the C termini of the overlapping genes A and B in bacteriophage φX174

• Biology, Chemistry
Nature
• 1977
Sequence determination of mutants in genes A and B confirms that these constitute another pair of genes in φX174 which are translated from the same DNA sequence using different reading frames.

### Physical structure of the replication origin of bacteriophage lambda.

• Biology
Science
• 1977
The nucleotide sequence of part of the replication region of wild-type bacteriophage lambda and of four mutants defective in the origin of DNA replication (ori-) has been determined and contains a number of unusual features.

### Sequence of promoter for coat protein gene of bacteriophage fd

• Biology
Nature
• 1976
The nucleotide sequence in the promoter region for the coat protein gene of phage fd has been determined and it is indicated that the longest sequence common to both was T AT AAT in the region in which RNA polymerase forms a stable initiation complex.