Nucleotide sequence of an avian sarcoma virus oncogene (src) and proposed amino acid sequence for gene product

@article{Czernilofsky1980NucleotideSO,
  title={Nucleotide sequence of an avian sarcoma virus oncogene (src) and proposed amino acid sequence for gene product},
  author={A. P. Czernilofsky and Arthur D. Levinson and Harold Varmus and J. M. Bishop and E. Tischer and HOWARD M. Goodman},
  journal={Nature},
  year={1980},
  volume={287},
  pages={198-203}
}
The transforming gene (src) of avian sarcoma virus (ASV) and adjacent regions of the viral genome have been isolated by molecular cloning of viral DNA. Their nucleotide sequence encompasses the whole of src and the portion of the gene env that encodes gp 37, one of two glycoproteins found in the viral envelope. Src encodes a single, hydrophobic protein with structural features that conform to previous descriptions of the gene product (pp60src). It appears that a single viral protein is… Expand
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References

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Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein
TLDR
It is concluded that both the protein kinase and pp60 are encoded in src, and that the enzymatic activity may be an intrinsic property of pp60. Expand
The size and genetic composition of virus-specific RNAs in the cytoplasm of cells producing avian sarcoma-leukosis viruses
TLDR
A model for viral gene expression is proposed which conforms to previous suggestions that eucaryotic cells initiate translations only at the 5′ termini of messenger RNAs. Expand
The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane.
TLDR
It is found that both pp60src and pp60proto-src were associated with the plasma membrane as active protein kinases and could be recovered efficiently only by disrupting the membranes with nonionic detergent. Expand
Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation
TLDR
The avian sarcoma virus (ASV) protein responsible for cellular transformation in vitro and sarcomagenesis in animals was studied structurally with special reference to the sites of phosphorylation on the polypeptide, finding that pp60src contained two major sites ofosphorylation, one involving phosphoserine and the other involving phosphothreonine and possible addtional minor sites. Expand
A normal cell protein similar in structure and function to the avian sarcoma virus transforming gene product.
TLDR
This report extends the previous studies concerning the identification and characterization of a protein from normal cells that is closely related to the avian sarcoma virus (ASV) transforming gene product pp60src to have an associated protein kinase activity analogous to that previously described for the viral pp60 src. Expand
Mapping unintegrated avian sarcoma virus DNA: Termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA
Three major species of viral DNA have been observed in cells infected by retroviruses: a linear, double-stranded copy of a subunit of viral RNA; closed circular DNA; and proviral DNA insertedExpand
Recombination between viral and cellular sequences generates transforming sarcoma virus.
TLDR
Results indicate that RNA sequences that rASVs have acquired from cells in the process of conversion from td virus to transforming virus are mapped within the src region and segregate with the transforming function. Expand
At least 104 nucleotides are transposed from the 5′ terminus of the avian sarcoma virus genome to the 5′ termini of smaller viral mrnas
TLDR
It is concluded that a sequence of more than 100 nucleotides is transposed from the 5' end of the ASV genome to the5' termini of smaller viral RNAs during the genesis of these RNAs. Expand
The nucleotide sequence of an untranslated but conserved domain at the 3' end of the avian sarcoma virus genome
TLDR
The sequence of over 900 nucleotides at the 3' end of the genome of the Schmidt-Ruppin subgroup A strain of avian sarcoma virus probably does not encode a protein, but its sequence may nevertheless serve several essential functions in viral replication. Expand
Subgenomic, cellular Rous sarcoma virus RNAs contain oligonucleotides from the 3′ half and the 5′ terminus of virion RNA
THE genome of Rous sarcoma virus (RSV) is a 30-40S RNA of 10,000 nucleotides coding for four known genes1 which map in the order 5′-gag, pol, env, src, poly(A)-3′ (refs 2, 3). This 30-40S RNA servesExpand
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