Nucleotide sequence of an avian sarcoma virus oncogene (src) and proposed amino acid sequence for gene product

  title={Nucleotide sequence of an avian sarcoma virus oncogene (src) and proposed amino acid sequence for gene product},
  author={A. P. Czernilofsky and Arthur D. Levinson and Harold Varmus and J. M. Bishop and E. Tischer and HOWARD M. Goodman},
The transforming gene (src) of avian sarcoma virus (ASV) and adjacent regions of the viral genome have been isolated by molecular cloning of viral DNA. Their nucleotide sequence encompasses the whole of src and the portion of the gene env that encodes gp 37, one of two glycoproteins found in the viral envelope. Src encodes a single, hydrophobic protein with structural features that conform to previous descriptions of the gene product (pp60src). It appears that a single viral protein is… 

Nucleotide sequence of avian sarcoma virus UR2 and comparison of its transforming gene with other members of the tyrosine protein kinase oncogene family

The deduced amino acid sequence for the UR2 transforming protein P68 gives a molecular weight of 61,113 and shows that it is closely related to the oncogene family coding for tyrosine protein kinases.

Nucleotide sequence and formation of the transforming gene of a mouse sarcoma virus

The predicted amino acid sequence of the mouse sarcoma virus transforming gene was found to share considerable homology with the proposed amino acid sequences of the avian sarcomA virus oncogene (src) product.

DNA sequence of the viral and cellular src gene of chickens. 1. Complete nucleotide sequence of an EcoRI fragment of recovered avian sarcoma virus which codes for gp37 and pp60src

The entire nucleotide sequence of a 3.1-kilobase EcoRI DNA fragment of molecularly cloned recovered avian sarcoma virus DNA is determined and amino acid sequences predicted differ significantly from the amino acid sequence predicted previously for the Schmidt-Ruppin strain of Rous sarcomA virus.

Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus

From the complete nucleotide sequence of the genome of the avian sarcoma virus Y73, predicted amino acid sequence of p90gag-yes, the product of the transforming gene, suggests that the cellular counterparts of the two (c-yes and c-src) originated from a common prototype sequence.

Activation of the cellular src gene by transducing retrovirus

The mechanism of c-src transduction and the structural changes necessary for pp60c-src activation are discussed.

DNA sequence of the viral and cellular src gene of chickens. II. Comparison of the src genes of two strains of avian sarcoma virus and of the cellular homolog

The results confirm the cellular origin of the src sequences of rASVs and provide information about the possible sites of the recombination.



The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane.

It is found that both pp60src and pp60proto-src were associated with the plasma membrane as active protein kinases and could be recovered efficiently only by disrupting the membranes with nonionic detergent.

Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation

The avian sarcoma virus (ASV) protein responsible for cellular transformation in vitro and sarcomagenesis in animals was studied structurally with special reference to the sites of phosphorylation on the polypeptide, finding that pp60src contained two major sites ofosphorylation, one involving phosphoserine and the other involving phosphothreonine and possible addtional minor sites.

A normal cell protein similar in structure and function to the avian sarcoma virus transforming gene product.

This report extends the previous studies concerning the identification and characterization of a protein from normal cells that is closely related to the avian sarcoma virus (ASV) transforming gene product pp60src to have an associated protein kinase activity analogous to that previously described for the viral pp60 src.

Recombination between viral and cellular sequences generates transforming sarcoma virus.

Results indicate that RNA sequences that rASVs have acquired from cells in the process of conversion from td virus to transforming virus are mapped within the src region and segregate with the transforming function.

Subgenomic, cellular Rous sarcoma virus RNAs contain oligonucleotides from the 3′ half and the 5′ terminus of virion RNA

THE genome of Rous sarcoma virus (RSV) is a 30-40S RNA of 10,000 nucleotides coding for four known genes1 which map in the order 5′-gag, pol, env, src, poly(A)-3′ (refs 2, 3). This 30-40S RNA serves

Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine

It is reported here that the soluble phosphotransferase activity of partially purified pp60src results in the phosphorylation of exclusively tyrosine residues in a variety of proteins that serve as substrates.