Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2)

  title={Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2)},
  author={Kenneth E. Narva and Jerald S. Feitelson},
  journal={Journal of Bacteriology},
  pages={326 - 333}
Previous genetic evidence suggested that the redD gene product might be involved in the regulation of undecylprodigiosin (Red) biosynthesis in Streptomyces coelicolor. The redD+ gene was subcloned on a 2.2-kilobase-pair restriction fragment from the S. coelicolor redCD region by complementation of S. coelicolor JF1 (redD42). The DNA sequence of the 2.2-kilobase-pair redD-complementing region was determined, and the redD coding sequence was identified by computer analysis and deletion subcloning… 

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Computer-assisted analysis of the DNA sequence revealed three putative open reading frames (ORFs), ORF1, ORF2, and ORF3 that strongly resembled the relA and spoT gene products from Escherichia coli and the homologs from Vibrio sp.

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