Nucleotide sequence and secondary structure of potato spindle tuber viroid

@article{Gross1978NucleotideSA,
  title={Nucleotide sequence and secondary structure of potato spindle tuber viroid},
  author={H. J. Gross and Horst Domdey and Christine Lossow and P Jank and Manfred Raba and Heidemarie Alberty and Heinz Ludwig S{\"a}nger},
  journal={Nature},
  year={1978},
  volume={273},
  pages={203-208}
}
The viroid of the potato spindle tuber disease (PSTV) is a covalently closed ring of 359 ribonucleotides. As a result of intramolecular base pairing, a serial arrangement of double-helical sections and internal loops form a unique rod-like secondary structure. PSTV is the first pathogen of a eukaryotic organism for which the complete molecular structure has been established. 
Sequence homology between potato spindle tuber viroid and U3B snRNA
TLDR
A strong relation in primary structure between PSTV and U3B snRNA is demonstrated by statistical analysis and is involved in the PSTV molecule unique sites which, if depicted in terms of the secondary structure of the circular PSTV molecules, reveal a conspicuous regularity in their location. Expand
Potato spindle tuber viroid (PSTVd)
TLDR
The present knowledge about functional motifs in PSTVd and their functional relationship to replication, processing, transport, and cause of symptoms is summarized. Expand
Existence in vivo of the loop E motif in potato spindle tuber viroid RNA
TLDR
It is reported that PSTVd (+) RNA also forms the loop E in vivo, providing strong support for the physiological relevance of this structural motif, which is involved in a wide range of functions including replication, host specificity and pathogenesis. Expand
Processing of Potato Spindle Tuber Viroid RNAs in Yeast, a Nonconventional Host
TLDR
It is concluded that PSTVd RNA is opportunistic and may use different processing pathways in different hosts, resulting in various susceptibilities to degradation versus processing. Expand
Genetic variability of potato spindle tuber viroid RNA replicon.
TLDR
The genetic continuity of the potato spindle tuber viroid genome was analysed after infection of tomato plants with cloned cDNAs of parental strains and surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected. Expand
Structure and function of viroids
TLDR
A hypothesis is proposed in which viroids interfere with the splicing process leading to a pathogenic misregulation of mRNA processing, and the suggestion that viroid RNA has features similar to DNA has been supported. Expand
Structure and function of viroids
Viroids are an independent class of plant pathogens which are distinguished from viruses by the absence of a protein coat and by their unusually small size. They are single-stranded circular RNAsExpand
Cell-Free Circularization of Viroid Progeny RNA by an RNA Ligase from Wheat Germ
TLDR
Linear, potato spindle tuber viroid RNA has been used as a substrate for an RNA ligase purified from wheat germ, suggesting cleavage followed by RNA ligation by a cellular enzyme may be a normal step in the viroid life cycle and may also reflect cellular events. Expand
Oligomeric forms of potato spindle tuber viroid (PSTV) and of its complementary RNA are present in nuclei isolated from viroid-infected potato cells
TLDR
Two oligomeric forms of PSTV are detected, which correspond in size to RNA strands of approximately two and three times viroid unit-length, which must be considered as the precursors os the circular and linear (+)PSTV monomers accumulating in the cell nucleus. Expand
Potato spindle tuber viroid: the simplicity paradox resolved?
  • R. Owens
  • Biology, Medicine
  • Molecular plant pathology
  • 2007
TAXONOMY Potato spindle tuber viroid (PSTVd) is the type species of the genus Posipiviroid, family Pospiviroidae. An absence of hammerhead ribozymes and the presence of a 'central conserved region'Expand
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References

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Size and secondary structure of potato spindle tuber viroid.
TLDR
The structure and size of potato spindle tuber viroid (PSTV) were investigated by electron microscopy and it was found that both components are sensitive to digestion by pancreatic ribonuclease but are resistant to snake venom phosphodiesterase. Expand
Separation and infectivity of circular and linear forms of potato spindle tuber viroid.
TLDR
Potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea and both circular and linear molecules were found to be infectious. Expand
Comparative oligonucleotide fingerprints of three plant viroids.
TLDR
These three plant viroids differ significantly from each other as judged from their oligonucleotide patterns, which supports the concept of individual viroid species. Expand
Potato spindle tuber viroid. XII. An investigation of viroid RNA as a messenger for protein synthesis.
Abstract The ability of potato spindle tuber viroid (PSTV) RNA to act as a messenger RNA for protein synthesis has been investigated in several cell-free protein synthesizing systems. Extracts fromExpand
Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene
TLDR
The complete, primary chemical structure of a viral genome has now been established and biological properties, such as ribosome binding and codon interactions can now be discussed on a molecular basis. Expand
Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures.
TLDR
Viroids are uncoated infectious RNA molecules pathogenic to certain higher plants and exhibit high thermal stability, cooperativity, and self-complementarity resulting in a rod-like native structure. Expand
Functional distinctions between the ribonucleic acids from citrus exocortis viroid and plant viruses: cell-free translation and aminoacylation reactions.
Abstract A low molecular weight RNA having tRNA-like physical characteristics has previously been shown to be the pathogen causing citrus exocortis disease. The ability of this RNA to serve as aExpand
Studies on the primary and secondary structure of potato spindle tuber viroid: products of digestion with ribonuclease A and ribonuclease T1, and modification with bisulfite.
TLDR
The detailed analysis of the bisulfite-catalized modification of cytidine to uridine in PSTV revealed a higher reactivity for the majority of the cytidines than would be expected for a perfect helix, which provides additional evidence for defects in the helical secondary structure of PSTV. Expand
Potato spindle tuber "virus". IV. A replicating, low molecular weight RNA.
TLDR
It is shown that the infectious RNA occurs in the form of several species with molecular weights ranging from 2.5 × 10 4 to 1.1 × 10 5 daltons, and the term “viroid” is proposed to designate potato spindle tuber “virus” RNA and other RNAs with similar properties. Expand
Nucleotide sequence of bacteriophage φX174 DNA
TLDR
The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs. Expand
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