Nucleotide sequence and secondary structure of potato spindle tuber viroid

@article{Gross1978NucleotideSA,
  title={Nucleotide sequence and secondary structure of potato spindle tuber viroid},
  author={H. J. Gross and Horst Domdey and Christine Lossow and Peter Jank and Manfred Raba and Heidemarie Alberty and Heinz Ludwig S{\"a}nger},
  journal={Nature},
  year={1978},
  volume={273},
  pages={203-208}
}
The viroid of the potato spindle tuber disease (PSTV) is a covalently closed ring of 359 ribonucleotides. As a result of intramolecular base pairing, a serial arrangement of double-helical sections and internal loops form a unique rod-like secondary structure. PSTV is the first pathogen of a eukaryotic organism for which the complete molecular structure has been established. 

Potato spindle tuber viroid (PSTVd)

The present knowledge about functional motifs in PSTVd and their functional relationship to replication, processing, transport, and cause of symptoms is summarized.

Existence in vivo of the loop E motif in potato spindle tuber viroid RNA

It is reported that PSTVd (+) RNA also forms the loop E in vivo, providing strong support for the physiological relevance of this structural motif, which is involved in a wide range of functions including replication, host specificity and pathogenesis.

Processing of Potato Spindle Tuber Viroid RNAs in Yeast, a Nonconventional Host

It is concluded that PSTVd RNA is opportunistic and may use different processing pathways in different hosts, resulting in various susceptibilities to degradation versus processing.

Genetic variability of potato spindle tuber viroid RNA replicon.

The genetic continuity of the potato spindle tuber viroid genome was analysed after infection of tomato plants with cloned cDNAs of parental strains and surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected.

Structure and function of viroids

A hypothesis is proposed in which viroids interfere with the splicing process leading to a pathogenic misregulation of mRNA processing, and the suggestion that viroid RNA has features similar to DNA has been supported.

Structure and function of viroids

A hypothesis is proposed in which viroids interfere with the splicing process leading to a pathogenic misregulation of mRNA processing, supported by the finding that they are replicated in vitro by the DNA-dependent RNA polymerase II of the host plant.

Cell-Free Circularization of Viroid Progeny RNA by an RNA Ligase from Wheat Germ

Linear, potato spindle tuber viroid RNA has been used as a substrate for an RNA ligase purified from wheat germ, suggesting cleavage followed by RNA ligation by a cellular enzyme may be a normal step in the viroid life cycle and may also reflect cellular events.

Oligomeric forms of potato spindle tuber viroid (PSTV) and of its complementary RNA are present in nuclei isolated from viroid-infected potato cells

Two oligomeric forms of PSTV are detected, which correspond in size to RNA strands of approximately two and three times viroid unit-length, which must be considered as the precursors os the circular and linear (+)PSTV monomers accumulating in the cell nucleus.

Potato spindle tuber viroid: the simplicity paradox resolved?

  • R. Owens
  • Biology
    Molecular plant pathology
  • 2007
TAXONOMY Potato spindle tuber viroid (PSTVd) is the type species of the genus Posipiviroid, family Pospiviroidae. An absence of hammerhead ribozymes and the presence of a 'central conserved region'
...

References

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Separation and infectivity of circular and linear forms of potato spindle tuber viroid.

Potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea and both circular and linear molecules were found to be infectious.

Comparative oligonucleotide fingerprints of three plant viroids.

These three plant viroids differ significantly from each other as judged from their oligonucleotide patterns, which supports the concept of individual viroid species.

Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene

The complete, primary chemical structure of a viral genome has now been established and biological properties, such as ribosome binding and codon interactions can now be discussed on a molecular basis.

Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures.

Viroids are uncoated infectious RNA molecules pathogenic to certain higher plants and exhibit high thermal stability, cooperativity, and self-complementarity resulting in a rod-like native structure.

Studies on the primary and secondary structure of potato spindle tuber viroid: products of digestion with ribonuclease A and ribonuclease T1, and modification with bisulfite.

The detailed analysis of the bisulfite-catalized modification of cytidine to uridine in PSTV revealed a higher reactivity for the majority of the cytidines than would be expected for a perfect helix, which provides additional evidence for defects in the helical secondary structure of PSTV.

Nucleotide sequence of bacteriophage φX174 DNA

The sequence identifies many of the features responsible for the production of the proteins of the nine known genes of the organism, including initiation and termination sites for the proteins and RNAs.

Nucleotide sequence of the gene for the major structural protein of SV40 virus.

The sequence of the portion of Simian Virus 40 that codes for the major structural protein of the virus is determined and it is shown that there is 2 to 1 preference for uridylic acid in the third position of codons.