Nucleotide sequence and mutational analysis of an immunity repressor gene from Bacillus subtilis temperate phage phi 105.

@article{Dhaese1985NucleotideSA,
  title={Nucleotide sequence and mutational analysis of an immunity repressor gene from Bacillus subtilis temperate phage phi 105.},
  author={Patrick Dhaese and J. Seurinck and Bart JGL de Smet and Marc C E Van Montagu},
  journal={Nucleic acids research},
  year={1985},
  volume={13 15},
  pages={5441-55}
}
We have identified and sequenced a bacteriophage phi 105 gene encoding an immunity repressor, the first to be characterized from a temperate phage infecting a Gram-positive host. Using superinfection immunity as an assay for repressor function, the phi 105 repressor gene was located within a 740-bp PvuII-HindIII subfragment near the left end of the phi 105 EcoRI-F fragment. We show that the repressor is specified by the 5'-proximal coding sequence of a translationally overlapping gene pair… CONTINUE READING