Nucleotide sequence and functions of the oriT operon in IncI1 plasmid R64.

@article{Furuya1991NucleotideSA,
  title={Nucleotide sequence and functions of the oriT operon in IncI1 plasmid R64.},
  author={Nobuhisa Furuya and T Nisioka and Teruya Komano},
  journal={Journal of bacteriology},
  year={1991},
  volume={173 7},
  pages={2231-7}
}
Two transfer genes of IncI1 plasmid R64, tentatively designated nikA and nikB, were cloned and sequenced. They are located adjacent to the origin of transfer (oriT) and appear to be organized into an operon, which we call the oriT operon. On the basis of the DNA sequence, nikA and nikB were concluded to encode proteins with 110 and 899 amino acid residues, respectively. Complementation analysis indicated that these two genes are indispensable for the transfer of R64 but are not required for the… CONTINUE READING