Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA.

@article{Zain1979NucleotideSA,
  title={Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA.},
  author={Saeeda Zain and Joe Sambrook and R. J. Roberts and Walter Keller and M Fried and Ashley R. Dunn},
  journal={Cell},
  year={1979},
  volume={16 4},
  pages={851-61}
}
Fiber mRNA of adenovirus 2 has been used as a template for RNA-dependent DNA polymerase. The resulting cDNA/RNA hybrids have been inserted at the Pst I site of the plasmid vector pBR322 after A:T tailing. One recombinant plasmid, pJAW 43, has been characterized in detail and shown to contain sequences from the main body of fiber mRNA, the three leaders common to most late adenoviral mRNAs and a fourth leader found in some species of fiber mRNA. The complete DNA sequence of the leader region has… CONTINUE READING