Nucleotide sequence analysis of the complement resistance gene from plasmid R100.

@article{Ogata1982NucleotideSA,
  title={Nucleotide sequence analysis of the complement resistance gene from plasmid R100.},
  author={Ronald T. Ogata and Carole Winters and Robert Paul Levine},
  journal={Journal of bacteriology},
  year={1982},
  volume={151 2},
  pages={819-27}
}
The multiple antibiotic resistance plasmid R100 renders Escherichia coli resistant to the bactericidal action of serum complement. We constructed a plasmid (pOW3) consisting of a 1,900-base-pair-long restriction fragment from R100 joined to a 2,900-base-pair-long fragment of pBR322 carrying ampicillin resistance. E. coli strains carrying pOW3 or R100 were up to 10,000-fold less sensitive to killing by serum complement than were plasmid-free bacteria or bacteria carrying pBR322. Nucleotide… CONTINUE READING

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