Nucleotide requirements for activated RNA polymerase II open complex formation in vitro.

@article{Jiang1995NucleotideRF,
  title={Nucleotide requirements for activated RNA polymerase II open complex formation in vitro.},
  author={Yi Jiang and J. D. Gralla},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 3},
  pages={1277-81}
}
The role of nucleotides in activated RNA polymerase II transcription was studied. Permanganate footprinting confirmed that there is a strict nucleotide requirement for forming open promoter complexes that cannot be overcome by the addition of a dinucleotide primer corresponding to the start site sequence. However, higher concentrations of other nucleoside triphosphates can substitute for ATP in catalyzing open complex formation. Opening catalyzed by these nucleotides is inhibited by the ATP… CONTINUE READING
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