Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster

  title={Nucleotide polymorphism at the alcohol dehydrogenase locus of Drosophila melanogaster},
  author={M. Kreitman},
The sequencing of eleven cloned Drosophila melanogaster alcohol dehydrogenase (Adh) genes from five natural populations has revealed a large number of previously hidden polymorphisms. Only one of the 43 polymorphisms results in an amino acid change, the one responsible for the two electrophoretic variants (fast, Adh-f, and slow, Adh-s) found in nearly all natural populations. The implication is that most amino acid changes in Adh would be selectively deleterious. 
Molecular similarity of Drosophila melanogaster alcohol dehydrogenase thermostable alleles from populations on different continents
Allele specific oligonucleotide probes have been used to show that DNA, amplified by the polymerase chain reaction, from eleven thermostable Adh alleles extracted from populations on differentExpand
The population genetics of alcohol dehydrogenase activity in Drosophila melanogaster
Between and within population variation in ADH activity and AD H protein in flies in the wild is mainly due to the relative frequencies of AdhF and Adhs. Expand
Persistence of an alcohol dehydrogenase thermostable variant in a natural population of Drosophila melanogaster.
A temporal survey of the alcohol dehydrogenase locus in a natural population of Drosophila melanogaster from the Canary Islands has revealed the existence of an electrophoretic Fast thermostableExpand
Conservation and change in the DNA sequences coding for alcohol dehydrogenase in sibling species of Drosophila
The DNA sequences of the alcohol dehydrogenase (Adh) genes of four very closely related species of Drosophila show that the rates of nucleotide change vary greatly in different functional domains ofExpand
Adh Nucleotide Variation in Drosophila willistoni: High Replacement Polymorphism in an Electrophoretically Monomorphic Protein
DNA sequence analysis of 18 alleles throughout the distribution of the species has revealed six replacement polymorphisms and the ratio of replacement to silent polymorphisms is higher in D. willistoni than in any other Drosophila species studied for Adh nucleotide variation. Expand
Allele-specific population structure of Drosophila melanogaster alcohol dehydrogenase at the molecular level.
The results suggest that the current range of Fast and In(2L)t Slow haplotypes is recent and that an older genetic differentiation between populations was followed by allele-specific gene flow. Expand
Molecular analysis of a spontaneous insertion mutation near the alcohol dehydrogenase gene of Drosophila melanogaster.
The structural integrity of the Adh gene in several isogenic lines of Drosophila melanogaster was tested by Southern blot analysis using a 4.75 kilobase (kb) genomic clone of Alcohol dehydrogenaseExpand
Molecular population genetics of an electrophoretically monomorphic protein in the alcohol dehydrogenase region of Drosophila pseudoobscura.
The results suggest that the ADH enzyme of D. pseudoobscura lacks amino acid polymorphisms because theneutral mutation rate of nonsynonymous sites is low, and the neutral mutation parameter for synonymous sites is heterogeneous between domains of the Adh region. Expand
Dehydrogenase Locus in the
Sequence variation among 10 alleles of the alcohol dehydrogenase (Adh) gene of the Hawaiian drosophilid D. mimica was analyzed with reference to the evolutionary history of the Hawaiian subgroup asExpand
An association between ADH protein levels and polymorphic nucleotide variation in the Adh gene of Drosophila melanogaster.
Southern analysis of the Adh region of 212 Drosophila melanogaster lines collected from the Tahbilk winery revealed linkage disequilibrium between a 37-bp insertion and the fast electrophoretic variant of alcohol dehydrogenase (ADH-F), suggesting that either polymorphic nucleotide-site variants positively associated with delta 2 on the second chromosome or delta 2 itself increases larval levels of ADH protein. Expand


Chemical basis of the electrophoretic variation observed at the alcohol dehydrogenase locus of Drosophila melanogaster.
It is concluded that electrophoretic methods should be capable of distinguishing many alleloenzymes which have identical isoelectric points without recourse to explanations involving conformational variability. Expand
Restriction map variation in the Adh region of Drosophila.
The relative distributions of restriction sites and insertion/ deletion variations among subpopulations and species suggest that the insertion/deletion variation may be mildly deleterious. Expand
An Electrophoretically Cryptic Alcohol Dehydrogenase Variant in Drosophila Melanogaster. 11. Post-Electrophoresis Heat-Treatment Screening of Natural Populations
The two common genetic variants of alcohol dehydrogenase in D. melanogaster, ADH-F and ADH-S, differ in substrate specificity and electrophoretic mobility. A third inherited variant, ADH-FCh.D., hasExpand
Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein.
The gene that codes for Drosophila alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase EC was identified in a bacteriophage lambda library of genomic Drosophila DNA by using ADH cDNAExpand
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
  • D. Goldberg
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1980
The alcohol dehydrogenase (ADH) gene (Adh) of Drosophila melanogaster was isolated by utilizing a mutant strain in which the Adh locus is deleted, and two intervening sequences were identified within Adh by S1 nuclease mapping experiments. Expand
The hypothesis that positive relationships between maximum temperature and Adhs and GpdhF gene frequencies underlie the latitudinal clines in North America is tested by examining these relationships on the continents of Asia, Europe, Australasia and North America. Expand
Spontaneous mutation rates at enzyme loci in Drosophila melanogaster
It is speculated that most viability and other fitness polygenes are located in controlling regions outside the structural genes of Drosophila melanogaster, which is much smaller than that estimated for viability poly Genes (0.12-0.17). Expand
Spontaneous Allozyme Mutations in DROSOPHILA MELANOGASTER: Rate of Occurrence and Nature of the Mutants.
After additional generations of accumulation of allozyme mutants, the 1,000 lines of Mukai and Cockerham (1977) were again screened for the same five loci and a qualitative analysis of the nulls provided evidence that most of the mutants recovered are due to base substitutions. Expand
The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5′ end in different developmental stages
The developmental specificity of Adh expression is seen to have a counterpart in the specificity of transcription initiation at the two separate promoter regions. Expand
A molecular approach to the study of genic heterozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura.
This study shows that there is a considerable amount of genic variation segregating in all of the populations studied and that the real variation in these populations must be greater than the authors are able to demonstrate. Expand