Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria.

@article{Nishiwaki2000NucleotideBA,
  title={Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria.},
  author={Taeko Nishiwaki and Hideo Iwasaki and Masahiro Ishiura and Takao Kondo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 1},
  pages={495-9}
}
A negative feedback control of kaiC expression by KaiC protein has been proposed to generate a basic oscillation of the circadian clock in the cyanobacterium Synechococcus sp. PCC 7942. KaiC has two P loops or Walker's motif As, that are potential ATP-/GTP-binding motifs and DXXG motifs conserved in various GTP-binding proteins. Herein, we demonstrate that in vitro KaiC binds ATP and, with lower affinity, GTP. Point mutation by site-directed mutagenesis of P loop 1 completely nullified the… CONTINUE READING

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