Nucleotide and deduced amino acid sequences of a high-molecular-mass subtilisin from an alkaliphilic Bacillus isolate.

@article{Ogawa2003NucleotideAD,
  title={Nucleotide and deduced amino acid sequences of a high-molecular-mass subtilisin from an alkaliphilic Bacillus isolate.},
  author={Akinori Ogawa and Nobuyuki Sumitomo and Mitsuyoshi Okuda and Katsuhisa Saeki and Shuji Kawai and Tohru Kobayashi and Susumu Ito},
  journal={Biochimica et biophysica acta},
  year={2003},
  volume={1624 1-3},
  pages={
          109-14
        }
}
A high-molecular-mass subtilisin was found in culture broth of the alkaliphilic Bacillus sp. strain KSM-KP43. The gene encoding the enzyme (FT protease) was determined using a mixed primer designed from the N-terminal amino acid (aa) sequence of the purified enzyme. The determined nucleotide sequence of the gene consisted of a 2427-bp open reading frame (ORF) that encoded a putative prepro-peptide (152 aa) and a mature enzyme (656 aa; 68,506 Da). The deduced aa of the mature enzyme revealed a… 
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