Nucleotide and deduced amino acid sequences of a high-molecular-mass subtilisin from an alkaliphilic Bacillus isolate.

  title={Nucleotide and deduced amino acid sequences of a high-molecular-mass subtilisin from an alkaliphilic Bacillus isolate.},
  author={Akinori Ogawa and Nobuyuki Sumitomo and Mitsuyoshi Okuda and Katsuhisa Saeki and Shuji Kawai and Tohru Kobayashi and Susumu Ito},
  journal={Biochimica et biophysica acta},
  volume={1624 1-3},
A high-molecular-mass subtilisin was found in culture broth of the alkaliphilic Bacillus sp. strain KSM-KP43. The gene encoding the enzyme (FT protease) was determined using a mixed primer designed from the N-terminal amino acid (aa) sequence of the purified enzyme. The determined nucleotide sequence of the gene consisted of a 2427-bp open reading frame (ORF) that encoded a putative prepro-peptide (152 aa) and a mature enzyme (656 aa; 68,506 Da). The deduced aa of the mature enzyme revealed a… 
A new subtilisin family: nucleotide and deduced amino acid sequences of new high-molecular-mass alkaline proteases from Bacillus spp.
Six genes encoding high-molecular-mass subtilisins (HMSs) of alkaliphilic Bacillus spp. were cloned and sequenced. Their open reading frames of 2,394–2,424 bp encoded prosubtilisins of 798–808 amino
Identification of a novel protease from the thermophilic Anoxybacillus kamchatkensis M1V and its application as laundry detergent additive
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Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes
The presence of 11 genes encoding subtilisin-like serine proteases was demonstrated by cloning from the genome of alkaliphilic Bacillus sp. strain KSM-LD1. This strain exoproduces the oxidatively
Intragenomic diversity of the V1 regions of 16S rRNA genes in high-alkaline protease-producing Bacillus clausii spp
B. clausii strains could clearly be divided into at least two subgroups based on the frequencies of the types of cloned V1 sequence, and was found to be in a different phylogenetic position from other high-alkaline protease-producing strains of B.Clausii.
Detergent alkaline proteases: enzymatic properties, genes, and crystal structures.
The large-scale production of a high-alkaline protease (M-protease) from alkaliphilic Bacillus clausii KSM-K16 is succeeded, and the enzyme has been introduced into compact heavy-duty laundry detergents.
Contribution of a Salt Bridge Triad to the Thermostability of a Highly Alkaline Protease from an Alkaliphilic Bacillus Strain*
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A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillus sp. SK37
The stability towards H2O2 and moderately halo- and thermo-tolerant properties of the AprX-SK37 enzyme are attractive for various biotechnological applications.


Nucleotide and Deduced Amino Acid Sequences of a New Subtilisin from an Alkaliphilic Bacillus Isolate
The deduced amino acid sequence of the mature enzyme (LD1) showed approximately 65% identity to those of subtilisins SprC and SprD from alkaliphilic Bacillus sp.
Cloning and characterization of the gene for an additional extracellular serine protease of Bacillus subtilis
During purification, this novel protease (Vpr) was found bound in a complex in the void volume after gel filtration chromatography, suggesting that Vpr undergoes C-terminal processing or proteolysis.
Novel oxidatively stable subtilisin-like serine proteases from alkaliphilic Bacillus spp.: enzymatic properties, sequences, and evolutionary relationships.
It is proposed that these novel proteases from alkaliphilic strains of Bacillus exhibiting resistance to oxidative inactivation be categorized as a new class of subtilisins, named oxidatively stable, alkaline protease.
Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16
Alkaline protease (EC activity, suitable for use in detergents, was detected in the alkaline culture medium of Bacillus sp. KSM-K16, which was originally isolated from soil. The enzyme,
A novel species of alkaliphilic Bacillus that produces an oxidatively stable alkaline serine protease
This novel strain produces a new class of protease, an oxidatively stable serine protease that is suitable for use in bleach-based detergents and is proposed as a novel species of alkaliphilic Bacillus.
Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39.
The nucleotide sequence of the subtilisin-encoding gene from the antarctic psychrotroph Bacillus TA39 was determined and Asp-145, His-185 and Ser-361 are the proposed catalytic residues of the active site.
Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis.
Out of 11 sigma factors which belong to the extracytoplasmic function family, 10 are unique to B. halodurans, suggesting that they may have a role in the special mechanism of adaptation to an alkaline environment.
The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
Bacillus subtilis is the best-characterized member of the Gram-positive bacteria, indicating that bacteriophage infection has played an important evolutionary role in horizontal gene transfer, in particular in the propagation of bacterial pathogenesis.
Signal Peptide-Dependent Protein Transport inBacillus subtilis: a Genome-Based Survey of the Secretome
The predictions and comparisons in this review pinpoint important differences as well as similarities between protein transport systems in B. subtilis and other well-studied organisms, such as Escherichia coli and the yeast Saccharomyces cerevisiae, which may serve as a lead for future research and applications.
Bacterial alkaline proteases: molecular approaches and industrial applications
The types and sources of proteases, protease yield-improvement methods, the use of new methods for developing novel proteases and applications of alkaline proteases in industrial sectors are discussed, with an overview on the use in the detergent industry.