Nucleotide- and activator-dependent structural and dynamic changes of arp2/3 complex monitored by hydrogen/deuterium exchange and mass spectrometry.

@article{Zencheck2009NucleotideAA,
  title={Nucleotide- and activator-dependent structural and dynamic changes of arp2/3 complex monitored by hydrogen/deuterium exchange and mass spectrometry.},
  author={Wendy D Zencheck and Hui Xiao and Brad J Nolen and Ruth Hogue Angeletti and Thomas D. Pollard and Steven C. Almo},
  journal={Journal of molecular biology},
  year={2009},
  volume={390 3},
  pages={
          414-27
        }
}
Arp2/3 complex plays a central role in the de novo nucleation of filamentous actin as branches on existing filaments. The complex must bind ATP, protein activators [e.g., Wiskott-Aldrich syndrome protein (WASp)], and the side of an actin filament to form a new actin filament. Amide hydrogen/deuterium exchange coupled with mass spectrometry was used to examine the structural and dynamic properties of the mammalian Arp2/3 complex in the presence of both ATP and the activating peptide segment from… CONTINUE READING
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