Nucleotide Sequence of the Gene Coding for the Bacteriophage MS2 Coat Protein

  title={Nucleotide Sequence of the Gene Coding for the Bacteriophage MS2 Coat Protein},
  author={W. Min Jou and Guy Haegeman and M. Ysebaert and Walter Charles Fiers},
By characterization of fragments, isolated from a nuclease digest of MS2 RNA, the entire nucleotide sequence of the coat gene was established. A “flower”-like model is proposed for the secondary structure. The genetic code makes use of 49 different codons to specify the sequence of the 129 amino-acids long coat polypeptide. 
Bacteriophage Ms2 RNA: nucleotide sequence of the end of the a protein gene and the intercistronic region.
The sequence of an RNA segment of 160 nucleotides preceding the coat protein initiator AUG has been established. It contains the genetic information for the last 45 amino-acids of the A or maturationExpand
A-Protein gene of bacteriophage MS2
The nucleotide sequence of the A-protein gene of bacteriophage MS2 has been determined and a model for its secondary structure is proposed. Also the amino acid sequence of the A-protein has beenExpand
Secondary structure and coding potential of the coat protein gene of bacteriophage MS2.
  • L. A. Ball
  • Biology, Medicine
  • Nature: New biology
  • 1973
The strongest evidence for the presence of base-paired secondary structure comes from the nucleotide sequences which have been determined for parts of the RNAs of bacteriophages MS21 and R172 and for a 6S RNA of bacteriaiophage λ3. Expand
Lysis gene expression of RNA phage MS2 depends on a frameshift during translation of the overlapping coat protein gene
Translation of the lysis cistron of MS2 RNA requires a frameshift of ribosomes reading the upstream coat protein mRNA. The out-of-phase fraction of ribosomes terminates at either one of two nonsenseExpand
Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene
The complete, primary chemical structure of a viral genome has now been established and biological properties, such as ribosome binding and codon interactions can now be discussed on a molecular basis. Expand
Gene F of bacteriophage phiX174. Correlation of nucleotide sequences from the DNA and amino acid sequences from the gene product.
Simultaneous work on amino acid sequences of X174 coat proteins showed that these nucleotide sequences are part of the F gene, coding for the largest coat protein of the bacteriophage. Expand
The nucleotide and amino acid sequences of the N (5′) terminal region of gene G of bacteriophage φX174
Abstract Gene G of bacteriophage φX174 DNA has been characterized by three techniques. (1) Transcription by RNA polymerase of appropriate DNA fragments from restriction enzyme digestion. (2) Use ofExpand
A sequence of 54 nucleotides from the A-protein cistron of coliphage-R17 RNA.
Three fragments from the A protein cistron of coliphage R17 ribonucleic acid have been found, purified and analysed from a partial T1 ribonuclease digest. A sequence of 54 nucleotides common to allExpand
The coding function of nucleotide sequences can be discerned by statistical analysis.
This analysis alone indicates that the genetic code is a non-overlapping triplet code and what the correct reading frame is, and the application of these methods to identify structure in sequences of unknown function is discussed. Expand
Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes
The nucleotide sequence has been determined for the first 53 bases of brome mosaic virus RNA4, the monocistronic messenger for brome mosaic virus coat protein. The sequence includes the binding siteExpand


Nucleotide Sequence from the Coat Protein Cistron of R17 Bacteriophage RNA
The sequence of fifty-seven nucleotides in the coat protein cistron of phage R17 RNA directly confirms the genetic code, shows that the code used by the phage is degenerate and suggests that highlyExpand
Nucleotide Sequence from the Polypeptide Chain Termination Region of the Coat Protein Cistron in Bacteriophage R17 RNA
The nucleotide sequence of a fragment of R17 RNA, which corresponds to the last six amino-acids of the bacteriophage coat protein, and extends on the 3′ side of the coat protein cistron forExpand
Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNA
The initiator regions of the three cistrons of R17 bacteriophage RNA have been isolated and sequenced. All three regions contain a UGA triplet as well as the expected AUG and two contain the sequenceExpand
Amino acid sequence of the f2 coat protein.
The complete amino acid sequence of the f2 coat protein has been determined by enzymatic fragmentation of the tryptic peptides, providing sufficient evidence for the proposed structure of the coat protein. Expand
Sequences of RNA fragments from the bacteriophage f2 coat protein cistron which differ from their R17 counterparts.
Two RNA fragments from a partial T1 ribonuclease digest of bacteriophage f2 RNA are isolated and their sequences are determined, including one which accounts for the only amino acid difference between the coat proteins of the two phages. Expand
Studies on the bacteriophage MS2. Nucleotide fragments from the coat protein cistron
The results further support the concept that the evolutionary process has taken advantage of the degeneracy of the genetic code in order to maximize secondary structure in this type of messenger RNA. Expand
The coat protein of the RNA bacteriophage MS2
Abstract The amino acid composition of the coat protein of the MS2 phage seems to be identical to that of M12. The f2 phage differs from these by one methionine to leucine exchange and the R17,Expand
Three complementation groups in bacteriophage R17.
  • G. Gussin
  • Biology, Medicine
  • Journal of molecular biology
  • 1966
Complementation between amber mutants of R17 reveals the existence of three phage cistrons, designated A, B and C, and two models for polarity based on the interaction between ribosomes and the phage message during protein synthesis are discussed. Expand
Nucleotide sequences from the low molecular weight ribosomal RNA of Escherichia coli.
Nucleotides from a ribonuclease digest of 32 P-labelled 5 † ribosomal RNA of Escherichia coli have been studied by two-dimensional ionophoresis on modified celluloses and the yields of the smaller nucleotides were reproduced and greater than 70%. Expand
Mutants of the bacteriophage f2. 8. Control mechanisms for phage-specific syntheses.
These results provide support for the existence of a phage-induced enzyme (enzyme I) which converts single-stranded viral RNA into a double-Stranded form late in infection. Expand