Nucleotide Sequence of a Yeast Tyrosine Transfer RNA

  title={Nucleotide Sequence of a Yeast Tyrosine Transfer RNA},
  author={James T. Madison and George A. Everett and H K Kung},
  pages={531 - 534}
The nucleotide sequence of a tyrosine transfer ribonucleic acid is described and compared to the known sequence of an alanine transfer RNA. It is possible to construct very similar base-paired models for the two molecules in spite of only limited similarities in sequences. The evidence indicates that the sequence containing guanosine, pseudouridine, and adenosine in the middle of the polynucleotide chain is the anticodon. 

Nucleotide Sequence of Escherichia coli Tyrosine Transfer Ribonucleic Acid

The information obtained suggests that the conformation of transfer ribonucleic acid molecules may be a greater contributing factor than a specific nucleotide sequence in the interaction of transfer ribs with its corresponding aminoacyl-transfer ribon nucleic acid synthetase.

Nucleotide Sequence of KB Cell 5S RNA

The nucleotide sequence of 5S RNA derived from KB carcinoma cell ribosomes has been determined and is the first time the sequence of an animal-cell RNA has be determined.

Structure of a Mammalian Serine tRNA

This work has compared the structure of a yeast transfer RNA with that of a mammalian one and found that yeast tyrosine tRNA lies between the dihydrouridine-containing and anticodon loops.

Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.

An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase,

Amber Suppression: a Nucleotide Change in the Anticodon of a Tyrosine Transfer RNA

In certain mutants a single base change alters the meaning of a messenger codon in such a way that, instead of spelling out an amino-acid, it spells out chain termination. Mutants in a quite

Aminoacylation of bisulphite-modified yeast tyrosine transfer RNA.

Four well-characterized modifications of yeast tRNATyr induced by reaction with bisulphite ions at pH 5.8 are described and three of the observed changes proved to be C-⇐U transitions produced by the series of reactions.

Structure and processing of yeast precursor tRNAs containing intervening sequences

A precursor of yeast tRNATyr is isolated and shown that it contains an intervening sequence identical to that found in the gene for tR NATyr, which is functionally inactive as it cannot be aminoacylated and the anticodon is not accessible for hydrogen bonding.

The Molecular Basis of Translation of the Genetic Message

This chapter discusses the way in which the genetic information stored in DNA1 is transmitted to the protein making machinery of the cell, so that a specific nucleotide sequence in DNA gives rise to a unique amino acid sequence in the polypeptide chains of proteins.

Structure of aspartate-tRNA from brewer's yeast.

The structure of aspartate-tRNA isolated from brewer's yeast is described and it is shown that it is one of the shortest tRNAs so far sequenced and has a pUp 5′ terminal sequence which has not yet been found in other sequenced t RNAs1–5.



Structure of a Ribonucleic Acid

The complete nucleotide sequence of an alanine transfer RNA, isolated from yeast, has been determined. This is the first nucleic acid for which the structure is known.

RNA codewords and protein synthesis, VII. On the general nature of the RNA code.

The template activities of twenty-six additional trinucleotides and nucleotide sequences suggested for RNA codons corresponding to amino acids are described and related to the nature of the RNA code.


  • J. PenswickR. W. Holley
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1965
cultures incubated at either 30 or 370 possess normal levels of aminoacyl-sRNA synthetase activity for 17 amino acids tested, but exhibit virtually no valyl sRNA synthetase activity, as measured by


The discovery that very brief treatment of the alanine RNA with RNase T1 cleaves the RNA. specifically into two large fragments' suggested that this enzyme might give additional specific cleavages if

The occurrence of methylated guanines in ribonucleic acids from several sources.

1methylguanine, 6-hydroxy-2-methylaminopurine and 2-dimethylamino-6-hydroxypurine are found to be constituents of ribonucleic acid from several different biological sources, by identification of the free bases, Ribonucleosides and ribon nucleotides from the ribon DNA acids.

Ribonucleases in Takadiastase

This work has found the existence of at least three enzymes attacking ribonucleic acid in takadiastase: one of them is more active at pH 7.5 and the others are moreactive at pH 4.5.