Nucleotide Sequence Database Comparison for Routine Dermatophyte Identification by Internal Transcribed Spacer 2 Genetic Region DNA Barcoding.

@article{Normand2018NucleotideSD,
  title={Nucleotide Sequence Database Comparison for Routine Dermatophyte Identification by Internal Transcribed Spacer 2 Genetic Region DNA Barcoding.},
  author={Anne-C{\'e}cile Normand and Ann Packeu and Carole Cassagne and Marijke Hendrickx and St{\'e}phane Ranque and Renaud Piarroux},
  journal={Journal of clinical microbiology},
  year={2018},
  volume={56 5}
}
Conventional dermatophyte identification is based on morphological features. However, recent studies have proposed to use the nucleotide sequences of the rRNA internal transcribed spacer (ITS) region as an identification barcode of all fungi, including dermatophytes. Several nucleotide databases are available to compare sequences and thus identify isolates; however, these databases often contain mislabeled sequences that impair sequence-based identification. We evaluated five of these databases… CONTINUE READING
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