Nucleotide/H(+)-dependent change in Mg2+ affinity at the ATPase inhibitory site of the mitochondrial F1-F0 ATP synthase.

The interactions between ADP and Mg2+ that result in the slowly reversible inhibition of the mitochondrial F1-F0 ATPase were studied. The Ki for the inhibitory Mg2+ is shown to be strongly dependent on the occupation of the nucleotide-binding sites. The inhibitory binding site for Mg2+ is not seen unless a stoichiometric amount of ADP is added [Biochem. J… (More)