Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones

@article{Lorch1987NucleosomesIT,
  title={Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones},
  author={Y. Lorch and Janice W. LaPointe and R. Kornberg},
  journal={Cell},
  year={1987},
  volume={49},
  pages={203-210}
}
  • Y. Lorch, Janice W. LaPointe, R. Kornberg
  • Published 1987
  • Biology, Medicine
  • Cell
    • Promoters were assembled in nucleosomes or ligated to nucleosomes and transcribed with SP6 RNA polymerase or with mammalian RNA polymerase II and accessory factors. Neither polymerase would initiate transcription at a promoter in a nucleosome, but once engaged in transcription, both polymerases were capable of reading through a nucleosome. In the course of readthrough transcription, the histones were displaced from the DNA, as shown by the exposure of restriction sites and by a shift of the… CONTINUE READING
    View on Elsevier
    doi.org
    441 Citations
    Highly Influencial Citations
    12
    Background Citations
    150
    Methods Citations
    7
    Results Citations
    2

    Topics from this paper.

    Explore Further: Topics Discussed in This Paper

      441 Citations
      Citation Type

      Citation Type

      Influence of chromatin folding on transcription initiation and elongation by RNA polymerase III.
      • 76
      A histone octamer can step around a transcribing polymerase without leaving the template
      • 221
      A nucleosome core is transferred out of the path of a transcribing polymerase
      • 119
      Transcription of dinucleosomal templates.
      • 4
      Nucleosome displacement in transcription.
      • 121
      • PDF
      Initiation on chromatin templates in a yeast RNA polymerase II transcription system.
      • 36
      • PDF
      DNA supercoiling facilitates the assembly of transcriptionally active chromatin on the adenovirus major late promoter.
      • 6
      Transcription through the nucleosome.
      • 3
      Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II
      • 271
      Transcription Through Chromatin
      • 4

      References

      SHOWING 1-10 OF 26 REFERENCES
      Studies on the mechanism of transcription of nucleosomal complexes.
      • 23
      Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro.
      • 64
      Transcription of in vitro assembled chromatin by Escherichia coli RNA polymerase.
      • 60
      Transcription of histone-covered T7 DNA by Escherichia coli RNA polymerase.
      • 53
      Binding of E. coli RNA polymerase to chromatin subunits.
      • M. Bustin
      • Biology, Medicine
      • Nucleic acids research
      • 1978
      • 14
      Studies on transcription of 3'-extended templates by mammalian RNA polymerase II. Parameters that affect the initiation and elongation reactions.
      • 48
      Effect of histone acetylation on structure and in vitro transcription of chromatin.
      • 94
      Transcription of nucleosomal DNA in SV40 minichromosomes by eukaryotic and prokaryotic RNA polymerases.
      • 10
      • PDF
      Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.
      • 127
      The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.
      • 17