Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones

@article{Lorch1987NucleosomesIT,
  title={Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones},
  author={Y. Lorch and Janice W. LaPointe and R. Kornberg},
  journal={Cell},
  year={1987},
  volume={49},
  pages={203-210}
}
  • Y. Lorch, Janice W. LaPointe, R. Kornberg
  • Published 1987
  • Biology, Medicine
  • Cell
  • Promoters were assembled in nucleosomes or ligated to nucleosomes and transcribed with SP6 RNA polymerase or with mammalian RNA polymerase II and accessory factors. Neither polymerase would initiate transcription at a promoter in a nucleosome, but once engaged in transcription, both polymerases were capable of reading through a nucleosome. In the course of readthrough transcription, the histones were displaced from the DNA, as shown by the exposure of restriction sites and by a shift of the… CONTINUE READING
    441 Citations

    Topics from this paper.

    A histone octamer can step around a transcribing polymerase without leaving the template
    • 221
    Transcription of dinucleosomal templates.
    • 4
    Nucleosome displacement in transcription.
    • 121
    • PDF
    Transcription through the nucleosome.
    • 3
    Transcription Through Chromatin
    • 4

    References

    SHOWING 1-10 OF 26 REFERENCES
    Studies on the mechanism of transcription of nucleosomal complexes.
    • 23
    Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro.
    • 64
    Binding of E. coli RNA polymerase to chromatin subunits.
    • M. Bustin
    • Biology, Medicine
    • Nucleic acids research
    • 1978
    • 14
    Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.
    • 127