Nucleosomes containing the histone variant H2A.Bbd organize only 118 base pairs of DNA.

@article{Bao2004NucleosomesCT,
  title={Nucleosomes containing the histone variant H2A.Bbd organize only 118 base pairs of DNA.},
  author={Yunhe Bao and Kasey L Konesky and Young-Jun Park and Simona Rosu and Pamela N Dyer and Danny Rangasamy and David J Tremethick and P. J. R. Laybourn and Karolin Luger},
  journal={The EMBO journal},
  year={2004},
  volume={23 16},
  pages={3314-24}
}
H2A.Bbd is an unusual histone variant whose sequence is only 48% conserved compared to major H2A. The major sequence differences are in the docking domain that tethers the H2A-H2B dimer to the (H3-H4)(2) tetramer; in addition, the C-terminal tail is absent in H2A.Bbd. We assembled nucleosomes in which H2A is replaced by H2A.Bbd (Bbd-NCP), and found that Bbd-NCP had a more relaxed structure in which only 118+/-2 bp of DNA is protected against digestion with micrococcal nuclease. The absence of… CONTINUE READING