Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions.

@article{Choi2018NucleosomesAD,
  title={Nucleosomes and DNA methylation shape meiotic DSB frequency in Arabidopsis thaliana transposons and gene regulatory regions.},
  author={Kyuha Choi and Xiaohui Zhao and Andrew J. Tock and Christophe Lambing and Charles J. Underwood and Thomas J. Hardcastle and He{\"i}di Serra and Juhyun Kim and Hyun Seob Cho and Jaeil Kim and Piotr A Ziolkowski and Nataliya E. Yelina and Ildoo Hwang and Robert A. Martienssen and Ian R. Henderson},
  journal={Genome research},
  year={2018},
  volume={28 4},
  pages={
          532-546
        }
}
Meiotic recombination initiates from DNA double-strand breaks (DSBs) generated by SPO11 topoisomerase-like complexes. Meiotic DSB frequency varies extensively along eukaryotic chromosomes, with hotspots controlled by chromatin and DNA sequence. To map meiotic DSBs throughout a plant genome, we purified and sequenced Arabidopsis thaliana SPO11-1-oligonucleotides. SPO11-1-oligos are elevated in gene promoters, terminators, and introns, which is driven by AT-sequence richness that excludes… CONTINUE READING