Nucleosome remodeling induced by RNA polymerase II: loss of the H2A/H2B dimer during transcription.

@article{Kireeva2002NucleosomeRI,
  title={Nucleosome remodeling induced by RNA polymerase II: loss of the H2A/H2B dimer during transcription.},
  author={Maria L. Kireeva and Wendy Walter and Vladimir Tchernajenko and Vladimir Bondarenko and Mikhail Kashlev and Vasily M. Studitsky},
  journal={Molecular cell},
  year={2002},
  volume={9 3},
  pages={541-52}
}
RNA polymerase II (Pol II) must transcribe genes in a chromatin environment in vivo. We examined transcription by Pol II through nucleosome cores in vitro. At physiological and lower ionic strengths, a mononucleosome imposes a strong block to elongation, which is relieved at increased ionic strength. Passage of Pol II causes a quantitative loss of one H2A/H2B dimer but does not alter the location of the nucleosome. In contrast, bacteriophage SP6 RNA polymerase (RNAP) efficiently transcribes… CONTINUE READING
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