Nucleosome acetylation sequencing to study the establishment of chromatin acetylation.

@article{Mittal2014NucleosomeAS,
  title={Nucleosome acetylation sequencing to study the establishment of chromatin acetylation.},
  author={Chitvan Mittal and Melissa J Blacketer and Michael A. Shogren-Knaak},
  journal={Analytical biochemistry},
  year={2014},
  volume={457},
  pages={51-8}
}
The establishment of posttranslational chromatin modifications is a major mechanism for regulating how genomic DNA is utilized. However, current in vitro chromatin assays do not monitor histone modifications at individual nucleosomes. Here we describe a strategy, nucleosome acetylation sequencing, that allows us to read the amount of modification at each nucleosome. In this approach, a bead-bound trinucleosome substrate is enzymatically acetylated with radiolabeled acetyl CoA by the SAGA… CONTINUE READING

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