Nucleosome acetylation sequencing to study the establishment of chromatin acetylation.

  title={Nucleosome acetylation sequencing to study the establishment of chromatin acetylation.},
  author={Chitvan Mittal and Melissa J Blacketer and Michael A. Shogren-Knaak},
  journal={Analytical biochemistry},
The establishment of posttranslational chromatin modifications is a major mechanism for regulating how genomic DNA is utilized. However, current in vitro chromatin assays do not monitor histone modifications at individual nucleosomes. Here we describe a strategy, nucleosome acetylation sequencing, that allows us to read the amount of modification at each nucleosome. In this approach, a bead-bound trinucleosome substrate is enzymatically acetylated with radiolabeled acetyl CoA by the SAGA… CONTINUE READING

From This Paper

Topics from this paper.
2 Citations
38 References
Similar Papers


Publications citing this paper.


Publications referenced by this paper.
Showing 1-10 of 38 references


  • A. Ranjan, G. Mizuguchi, +5 authors C. L. Woodcock
  • Wu, Nucleosome-free region dominates histone…
  • 2013
1 Excerpt

Nucleosome - free region dominates histone acetylation in targeting SWR 1 to promoters for H 2 A

  • G. Mizuguchi A. Ranjan, P. C. FitzGerald, +5 authors C. Wu
  • Z replacement , Cell
  • 2013

Kouzarides , Regulation of chromatin by histone modifications

  • T. A. J. Bannister
  • Cell Res .
  • 2011


  • Z. Nagy, A. Riss, C. Romier
  • le Guezennec, A.R. Dongre, M. Orpinell, J. Han, H…
  • 2009
1 Excerpt

Similar Papers

Loading similar papers…