Nucleoside triphosphate binding to DNA polymerase III holoenzyme of Escherichia coli. A direct photoaffinity labeling study.

Abstract

The physical basis of ATP binding and activation of DNA polymerase III holoenzyme was studied by an ultraviolet irradiation cross-linking technique. ATP and dATP were photocrosslinked to the alpha, tau, gamma, and delta subunits of holoenzyme; photocrosslinking of dATP was competitively inhibited by ATP. No photocrosslinking was observed with GTP or CTP… (More)

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Cite this paper

@article{Biswas1984NucleosideTB, title={Nucleoside triphosphate binding to DNA polymerase III holoenzyme of Escherichia coli. A direct photoaffinity labeling study.}, author={Subhasis B. Biswas and Arthur Kornberg}, journal={The Journal of biological chemistry}, year={1984}, volume={259 12}, pages={7990-3} }