Nucleoside diphosphate kinase from haloalkaliphilic archaeon Natronobacterium magadii: purification and characterization

@article{Polosina1998NucleosideDK,
  title={Nucleoside diphosphate kinase from haloalkaliphilic archaeon Natronobacterium magadii: purification and characterization},
  author={Yaroslava Y. Polosina and Ken F Jarrell and Oleg V. Fedorov and Alla S. Kostyukova},
  journal={Extremophiles},
  year={1998},
  volume={2},
  pages={333-338}
}
An ATP-binding protein from the haloalkaliphilic archaeon Natronobacterium magadii was purified and characterized by affinity chromatography on ATP-agarose and by fast protein liquid chromatography (FPLC) on a Mono Q column. The N-terminal 20 amino acid sequence of the kinase showed a strong sequence similarity of this protein with nucleoside diphosphate (NDP) kinases from different organisms and, accordingly, we believe that this protein is a nucleoside diphosphate kinase, an enzyme whose main… CONTINUE READING