Nucleoside-diphosphate kinase from Streptomyces coelicolor.

  title={Nucleoside-diphosphate kinase from Streptomyces coelicolor.},
  author={M Brodbeck and A Rohling and Wolfgang Wohlleben and Charles B. Thompson and U S{\"u}sstrunk},
  journal={European journal of biochemistry},
  volume={239 1},
Nucleoside-diphosphate (NDP) kinase was purified from crude extracts of Streptomyces coelicolor to over 90% homogeneity in a single step using an adenosine 3',5'-cyclic monophosphate (cAMP) binding column. The specific activity of protein in the fraction eluted with cAMP (400 U/mg) was about 3600-fold higher than that in the crude extract. This enzyme was autophosphorylated in the presence of [y-32P]ATP. The high-energy phosphoenzyme intermediate was stable in alkali and highly labile in acid… CONTINUE READING