Nucleoplasmic bridges and tailed nuclei are signatures of radiation exposure in Oreochromis mossambicus using erythrocyte micronucleus cytome assay (EMNCA)

  title={Nucleoplasmic bridges and tailed nuclei are signatures of radiation exposure in Oreochromis mossambicus using erythrocyte micronucleus cytome assay (EMNCA)},
  author={Sadasivam Anbumani and Mary N. Mohankumar},
  journal={Environmental Science and Pollution Research},
Gamma radiation-induced genetic perturbations in aquatic vertebrates is largely unknown at low-dose rate, especially in the wake of a nuclear disaster and/or other environmental outbreaks. Freshwater fish, Oreochromis mossambicus subjected to low-dose rate (2 mGy/min) at 2.5-, 5-, and 10-Gy doses, were analyzed for “exposure signatures” in blood samples drawn on days 3, 6, 12, 18, and 30, respectively. Significant dose-dependent increments in micronuclei frequency and other anomalies such as… 
Erythrocyte micronucleus cytome assay of 17 wild bird species from the central Monte desert, Argentina
The notion that the use of micronuclei frequency and other nuclear abnormalities in erythrocytes could be effectively applied to evaluate spontaneous or induced genetic instability in wild birds is supported.
Detection of Micronucleus, Nucleoplasmic Bridges, and Nuclear Buds Frequency in Peripheral Blood Lymphocytes of Cancer Patient Post-Radiation Fractionated
The induction of micronucleus, Nucleoplasmic Bridge, and Nuclear Buds in cancer patients after administration of fractionated radiation exposure total of 20 Gy showed that there was a statistically significant difference between the frequency of MN in the study group and controls, and demographic factors such as age can influence the appearance of MN, NPB, and NBUD.
The Frequency of Lymphocytes Containing Dumbbell-Shaped Nuclei Depends on Ionizing Radiation Dose and Correlates with Appearance of Chromosomal Aberrations
It is concluded that nuclear anomalies formed as a result of chromosomal aberrations arising in lymphocytes under the action of ionizing radiation are converted into dumbbell-shaped nuclei in vitro in the culture of lymphocytes in the cytochalasin block.
Basal levels of inorganic elements, genetic damages, and hematological values in captive Falco peregrinus
The basal frequency of micronuclei and other nuclear alterations in peripheral blood erythrocytes of Falco peregrinus is determined and reference values of cytogenetic damage in this species are obtained and could be useful for future comparisons in biomonitoring studies.
Nuclear Abnormalities of Lymphocytes as the Simplest Markers for Bioindication Test in Case of Mass Casualty Events Involving Radiation Exposure
This review observes the main types of nuclear anomalies of lymphocytes in the light of their common origin from dicentric chromosomes and recommends using these alterations in peripheral blood lymphocytes nuclei as the simplest biomarkers in the framework of bioindication tests when it is necessary to work with a large number of victims.
1 Индийский институт токсикологических исследований Совета научных и промышленных исследований (CSIRIITR) (30, Vishvigyan Bhavan, M.G. Marg, Lucknow, Uttarpradesh, 226001, India); 2
A cytogenetic study on the community members chronically exposed to elevated natural radiation in Mamuju: 8-shaped cells as an optional biomarker


Gamma radiation induced micronuclei and erythrocyte cellular abnormalities in the fish Catla catla.
Cytogenetic dose-response and adaptive response in cells of ungulate species exposed to ionizing radiation.
Long-term irradiation of a marine fish, the plaice Pleuronectes platessa: an assessment of the effects on size and composition of the testes and of possible genotoxic changes in peripheral erythrocytes.
  • J. Knowles
  • Environmental Science
    International journal of radiation biology
  • 1999
This is the first investigation of a marine fish and it indicates that plaice testes are probably more radiosensitive than those previously described in tropical fish and of a similar radiosensitivity to mammalian testes.
Impacts of Low-Dose Gamma-Radiation on Genotoxic Risk in Aquatic Ecosystems
It is postulate that radiation risk in the whole organism may also be lower because fish cells appear to be more radiation resistant than mammalian cells, and whole body studies designed to test effects with the specific aim of assessing relative risk between species are in process.
Dose‐ and time‐dependent responses for micronucleus induction by X‐rays and fast neutrons in gill cells of medaka (Oryzias latipes)
Results and other data support the conclusion that the medaka gill cell micronucleus assay is a reliable short‐term test for detecting potential inducers of DNA double‐strand breaks.
Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells.
The wealth of knowledge currently available that best explains the formation of these important nuclear anomalies that are commonly seen in cancer and are indicative of genome damage events that could increase the risk of developmental and degenerative diseases are summarized.