Nucleologenesis in the Caenorhabditis elegans Embryo

@inproceedings{Korekov2012NucleologenesisIT,
  title={Nucleologenesis in the Caenorhabditis elegans Embryo},
  author={Darina Kor{\vc}ekov{\'a} and Adri{\'a}na Gombitov{\'a} and Ivan Raska and Du{\vs}an Cmarko and Christian Lanct{\^o}t},
  booktitle={PloS one},
  year={2012}
}
In the Caenorhabditis elegans nematode, the oocyte nucleolus disappears prior to fertilization. We have now investigated the re-formation of the nucleolus in the early embryo of this model organism by immunostaining for fibrillarin and DAO-5, a putative NOLC1/Nopp140 homolog involved in ribosome assembly. We find that labeled nucleoli first appear in somatic cells at around the 8-cell stage, at a time when transcription of the embryonic genome begins. Quantitative analysis of radial positioning… CONTINUE READING
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