Nucleic acid sequence-based amplification assay for human papillomavirus mRNA detection and typing: evidence for DNA amplification.

@article{Boulet2010NucleicAS,
  title={Nucleic acid sequence-based amplification assay for human papillomavirus mRNA detection and typing: evidence for DNA amplification.},
  author={Ga{\"e}lle A V Boulet and Isabel M Micalessi and Caroline A J Horvath and Ina H. Benoy and Christophe E Depuydt and J. Bogers},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 7},
  pages={2524-9}
}
Human papillomavirus (HPV) E6/E7 mRNA has been proposed as a more specific marker for cervical dysplasia and cancer than HPV DNA. This study evaluated the RNA specificity of nucleic acid sequence-based amplification (NASBA)-based HPV detection using HPV DNA plasmids (HPV type 16 [HPV16], HPV18, HPV31, HPV33, and HPV45) and nucleic acid extracts of several cell lines, which were systematically subjected to enzymatic treatments with DNase and RNase. HPV plasmid dilutions (10(6) to 10(0) copies… CONTINUE READING

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