Nucleic acid quantity and quality from paraffin blocks: defining optimal fixation, processing and DNA/RNA extraction techniques.

@article{Turashvili2012NucleicAQ,
  title={Nucleic acid quantity and quality from paraffin blocks: defining optimal fixation, processing and DNA/RNA extraction techniques.},
  author={Gulisa Turashvili and Winnie Yang and Steven McKinney and Steve E Kalloger and Nadia Gale and Ying Hui Ng and Katie Chow and Lynda L Bell and Julie Lorette and M. Carrier and Margaret Ty Luk and S. Aparicio and David Huntsman and Stephen Yip},
  journal={Experimental and molecular pathology},
  year={2012},
  volume={92 1},
  pages={33-43}
}
Although the extraction and analysis of nucleic acids from formalin-fixed paraffin-embedded tissues is a routine and growing part of pathology practice, no generally accepted recommendations exist to guide laboratories in their selection of tissue fixation, processing and DNA/RNA extraction techniques. The aim of this study was to determine how fixation method and length, paraffin embedding, processing conditions and nucleic acid extraction methods affect quality and quantity of DNA and RNA… CONTINUE READING

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