Nucleic acid polymerases employ a general acid for nucleotidyl transfer

@inproceedings{Castro2009NucleicAP,
  title={Nucleic acid polymerases employ a general acid for nucleotidyl transfer},
  author={Christian Castro and Eric D. Smidansky and Jamie J Arnold and Kenneth R. Maksimchuk and Ibrahim Moustafa Moustafa and Akira Uchida and Matthias G{\"o}tte and William H. Konigsberg and Craig E Cameron},
  booktitle={Nature Structural &Molecular Biology},
  year={2009}
}
Nucleic acid polymerases catalyze the formation of DNA or RNA from nucleoside-triphosphate precursors. Amino acid residues in the active site of polymerases are thought to contribute only indirectly to catalysis by serving as ligands for the two divalent cations that are required for activity or substrate binding. Two proton-transfer reactions are necessary for polymerase-catalyzed nucleotidyl transfer: deprotonation of the 3′-hydroxyl nucleophile and protonation of the pyrophosphate leaving… CONTINUE READING
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