Nucleic acid affinity chromatography: preparation and characterization of double-stranded RNA agarose.

@article{Langland1995NucleicAA,
  title={Nucleic acid affinity chromatography: preparation and characterization of double-stranded RNA agarose.},
  author={Jeffrey Langland and Sherrie M Pettiford and Bertram L Jacobs},
  journal={Protein expression and purification},
  year={1995},
  volume={6 1},
  pages={25-32}
}
An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl). poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and… CONTINUE READING

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