Nucleic acid-8-methoxypsoralen crosslinks bind monoclonal anti-Z-DNA antibody.

  title={Nucleic acid-8-methoxypsoralen crosslinks bind monoclonal anti-Z-DNA antibody.},
  author={Z Arif and Rashid Ali},
  journal={Biochemistry and molecular biology international},
  volume={40 5},
  • Z Arif, Rashid Ali
  • Published 1996 in Biochemistry and molecular biology international
Native calf thymus DNA and poly(dA-dT).poly(dA-dT) were photo-adducted with 8-methoxypsoralen and characterized by thermal denaturation (Tm) and hydroxyapatite column chromatography. The data demonstrated the formation of interstrand photo-crosslinks. It has been shown by competition ELISA and band shift assays that crosslinked species of DNA-8-MOP and poly(dA-dT)-8-MOP photoadducts recognize previously defined monoclonal anti-Z-DNA antibody (Z22). The results indicate the possible presence of… CONTINUE READING