Nucleic Acid-Mediated Cleavage of M1 Gene of Influenza A Virus Is Significantly Augmented by Antisense Molecules Targeted to Hybridize Close to the Cleavage Site

  title={Nucleic Acid-Mediated Cleavage of M1 Gene of Influenza A Virus Is Significantly Augmented by Antisense Molecules Targeted to Hybridize Close to the Cleavage Site},
  author={Binod Kumar and Madhu Khanna and P. Kumar and Vikas Sood and Rajesh Vyas and A. C. Banerjea},
  journal={Molecular Biotechnology},
Influenza A virus genome segment 7 encodes protein M1, which is the matrix protein playing crucial role in the virus life cycle. Any antiviral strategy that aims at reducing, in particular, the expression of this genome segment should, in principle, reduce the infectivity of the virus. We developed a specific antiviral approach at the molecular level and designed several novel 10–23 DNAzymes (Dz) and hammerhead ribozymes (Rz), specifically targeted to cleave at the conserved domains of the… 

Sequence-specific cleavage of BM2 gene transcript of influenza B virus by 10-23 catalytic motif containing DNA enzymes significantly inhibits viral RNA translation and replication.

The findings suggest that the Dz molecule can be used as selective and effective inhibitor of viral RNA replication, and can be explored further for development of a potent therapeutic agent against influenza B virus infection.

Potent Intracellular Knock-Down of Influenza A Virus M2 Gene Transcript by DNAzymes Considerably Reduces Viral Replication in Host Cells

It is suggested that Dz may be used as potential inhibitor of viral RNA replication and can be explored further for development of an effective therapeutic agent against influenza infection.

RNA Secondary Structure as a First Step for Rational Design of the Oligonucleotides towards Inhibition of Influenza A Virus Replication

The vRNA structure-function relationship was focused on as well as the advantages of using secondary structure information in predicting therapeutic targets and the potential future of this field were presented.

A Conserved Secondary Structural Element in the Coding Region of the Influenza A Virus Nucleoprotein (NP) mRNA Is Important for the Regulation of Viral Proliferation

A bioinformatics analysis of segment 5, which encodes nucleoprotein, revealed a conserved structural motif in the (+)RNA that might be a candidate for oligonucleotide-based antiviral therapy.

Taming Influenza Virus: Role of Antisense Technology.

The present review consolidates the efforts of researchers worldwide in this particular field and discusses the future perspectives this technology holds, to tame this notorious virus.

DNA enzymes as potential therapeutics: towards clinical application of 10-23 DNAzymes

In comparison with antisense oligonucleotides and small interfering RNAs, Dzs do not usually show off-target effects due to their high specificity and lack of immunogenicity in vivo, so there is a good chance that a deoxyribozyme drug reaching the clinic in the near future.

Antisense technology as a potential strategy for the treatment of coronaviruses infection: With focus on COVID‐19

In this review, the potential applications of antisense technology for the treatment of coronaviruses and specifically COVID‐19 infection are summarized.



Ribozymes that cleave reovirus genome segment S1 also protect cells from pathogenesis caused by reovirus infection

Rzs, besides acting as antiviral agents, could be exploited as biological tools to delineate specific functions of target genes to reduce the infectivity of the virus.

DNA-enzyme-mediated cleavage of human immunodeficiency virus type 1 Gag RNA is significantly augmented by antisense-DNA molecules targeted to hybridize close to the cleavage site.

It was possible to enhance significantly the cleavage activity of poorly cleaving HIV-1 Gag-specific Dzs by using sequence-specific ODNs, and this combination of antisense and catalytic Dz will, in principle, result in more effective gene suppression that could be exploited for therapeutic purposes.

Inhibition of influenza A virus reproduction by a ribozyme targeted against PB1 mRNA.

Sequence-specific cleavage activities of DNA enzymes targeted against HIV-1 Gag and Nef regions.

The Gag DNA enzymes could be exploited in combination with other promising antiviral approaches and showed significant variation in their cleavage activities in the presence of varying concentration of Mg(2+) and did not interfere with the replication of a laboratory-adapted HIV-1 isolate under in vitro culture conditions.

Target sequence-specific inhibition of HIV-1 replication by ribozymes directed to tat RNA.

The importance of the first base pair distal to the XUY within helix I of the hammerhead structure for both in vitro and in vivo ribozyme activities is demonstrated and imply that the effectiveness of the anti-HIV-1 ribozymes against appropriate target sequences is due to their catalytic activities rather than any antisense effect.

siRNAs, ribozymes and RNA decoys in modeling stem cell-based gene therapy for HIV/AIDS.

These proof-of-concept studies demonstrated the utility of RNA-based anti-HIV constructs for gene therapy and described the newly described siRNAs.

Ribozyme mediated destruction of influenza A virus in vitro and in vivo

In vitro hammerhead and hairpin ribozymes designed to cleave viral RNA segment 5 of influenza A virus were functional under optimal in vitro conditions, but quantitative measurements indicate that the hammerhead ribozyme is considerably more efficient at this target site than the hairpinribozyme.

A general purpose RNA-cleaving DNA enzyme.

  • S. SantoroG. F. Joyce
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1997
An in vitro selection procedure was used to develop a DNA enzyme that can be made to cleave almost any targeted RNA substrate under simulated physiological conditions, and its activity is dependent on the presence of Mg2+ ion.