Nucleation of polar actin filament assembly by a positively charged surface

@article{Brown1979NucleationOP,
  title={Nucleation of polar actin filament assembly by a positively charged surface},
  author={Susan S. Brown and James A Spudich},
  journal={The Journal of Cell Biology},
  year={1979},
  volume={80},
  pages={499 - 504}
}
Polylysine-coated polystyrene beads can nucleate polar assembly of monomeric actin into filamentous form. This nucleation has been demonstrated by a combination of biochemical and structural experiments. The polylysine-coated beads accelerate the rate of actin assembly as detected by two different biochemical assays. Subsequent examination of the beads by electron microscopy reveals numerous actin filaments of similar length radiating from the beads. ATP promotes this bead-induced acceleration… CONTINUE READING