Nuclease activities of Moloney murine leukemia virus reverse transcriptase. Mutants with altered substrate specificities.

@article{Blain1993NucleaseAO,
  title={Nuclease activities of Moloney murine leukemia virus reverse transcriptase. Mutants with altered substrate specificities.},
  author={Stacy W. Blain and Stephen P Goff},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 31},
  pages={23585-92}
}
RNases H are traditionally thought to degrade RNA only in RNA-DNA hybrid form. We found that the wild-type Moloney murine leukemia virus (M-MuLV) reverse transcriptase (RT) was capable of degrading RNA in RNA-RNA duplexes as well as in RNA-DNA hybrids, as assayed by in situ gel techniques. Escherichia coli RNase H does not degrade the RNA-RNA duplex in this assay, while E. coli RNase III, a double-strand-specific ribonuclease, does. The apparent specific activity of M-MuLV RT on RNA-RNA… CONTINUE READING

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