Nuclease Stability of LNA Oligonucleotides and LNA-DNA Chimeras

  title={Nuclease Stability of LNA Oligonucleotides and LNA-DNA Chimeras},
  author={Miriam Frieden and Henrik Frydenlund Hansen and Troels Koch},
  journal={Nucleosides, Nucleotides \& Nucleic Acids},
  pages={1041 - 1043}
Abstract The stabilizing properties of LNA and α-l-LNA oligonucleotides against endo- and 3′-exonucleases have been evaluated. 

Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

It was found that the 5′-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°Nm, Phusion and HIV RT, and it was discovered that primer extension can be achieved using templates containing one α- L-L NA nucleotide.

LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA.

The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.

DNA and RNA derivatives to optimize distribution and delivery.

  • E. Wickstrom
  • Biology, Chemistry
    Advanced drug delivery reviews
  • 2015

Design of LNA probes that improve mismatch discrimination

Forescence experiments using 2-aminopurine suggest that LNA modifications enhance base stacking of perfectly matched base pairs and decrease stabilizing stacking interactions of mismatched base pairs, and new guidelines are suggested for design of LNA probes, which significantly improve mismatch discrimination in comparison with unmodified DNA probes.

The influence of locked nucleic acid residues on the thermodynamic properties of 2′-O-methyl RNA/RNA heteroduplexes

The influence of locked nucleic acid (LNA) residues on the thermodynamic properties of 2′-O-methyl RNA/RNA heteroduplexes is reported and an equation is proposed to approximate the stabilities of complementary duplexes formed with RNA when at least one 2-methyl nucleotide separates LNA nucleotides.

Analysis of in Situ LNA and DNA Hybridization Events on Microspheres.

It is found that the extent of hybridization between single-stranded, immobilized probes and soluble targets generally increases with target sequence length or with the incorporation of LNA nucleotides in one or both oligonucleotide strands involved in duplex formation.

Comparison of the fragmentation behavior of DNA and LNA single strands and duplexes.

While DNA-DNA duplexes dissociated easily into single stranded fragments, the high stability of DNA-LNA hybrids resulted in predominant fragmentation of the DNA part rather than the LNA, while base losses were only observed from the DNA single strand of the hybrid.

DNA triple helices: biological consequences and therapeutic potential.




LNA (locked nucleic acids): synthesis and high-affinity nucleic acid recognition

A novel class of nucleic acid analogues, termed LNA (locked nucleic acids), is introduced. Following the Watson–Crick base pairing rules, LNA forms duplexes with complementary DNA and RNA with

Potent and nontoxic antisense oligonucleotides containing locked nucleic acids.

It is demonstrated here that a high-affinity DNA analog, locked nucleic acid (LNA), confers several desired properties to antisense agents, and LNA/DNA copolymers exhibited potent antisense activity on assay systems as disparate as a G-protein-coupled receptor in living rat brain and an Escherichia coli reporter gene.

Nuclease stability of LNA oligonucleotides and LNA-DNA chimeras.

  • 2003

Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recogniton

  • Tetrahedron
  • 1998

Stability and structural features of the duplexes containing analogues with a fixed N-type conformation, 20-O,40-C-methyleneribonucleosides

  • Tetrahedron Lett. 1998,
  • 1998