Nuclease Stability of LNA Oligonucleotides and LNA-DNA Chimeras

@article{Frieden2003NucleaseSO,
  title={Nuclease Stability of LNA Oligonucleotides and LNA-DNA Chimeras},
  author={Miriam Frieden and Henrik Frydenlund Hansen and Troels Koch},
  journal={Nucleosides, Nucleotides \& Nucleic Acids},
  year={2003},
  volume={22},
  pages={1041 - 1043}
}
Abstract The stabilizing properties of LNA and α-l-LNA oligonucleotides against endo- and 3′-exonucleases have been evaluated. 

Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

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It was found that the 5′-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°Nm, Phusion and HIV RT, and it was discovered that primer extension can be achieved using templates containing one α- L-L NA nucleotide.

LNA (locked nucleic acid): high-affinity targeting of complementary RNA and DNA.

TLDR
The wide applicability of LNA oligonucleotides for gene silencing and their use for research and diagnostic purposes are documented in a number of recent reports, some of which are described herein.

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Design of LNA probes that improve mismatch discrimination

TLDR
Forescence experiments using 2-aminopurine suggest that LNA modifications enhance base stacking of perfectly matched base pairs and decrease stabilizing stacking interactions of mismatched base pairs, and new guidelines are suggested for design of LNA probes, which significantly improve mismatch discrimination in comparison with unmodified DNA probes.

The influence of locked nucleic acid residues on the thermodynamic properties of 2′-O-methyl RNA/RNA heteroduplexes

TLDR
The influence of locked nucleic acid (LNA) residues on the thermodynamic properties of 2′-O-methyl RNA/RNA heteroduplexes is reported and an equation is proposed to approximate the stabilities of complementary duplexes formed with RNA when at least one 2-methyl nucleotide separates LNA nucleotides.

Analysis of in Situ LNA and DNA Hybridization Events on Microspheres.

TLDR
It is found that the extent of hybridization between single-stranded, immobilized probes and soluble targets generally increases with target sequence length or with the incorporation of LNA nucleotides in one or both oligonucleotide strands involved in duplex formation.

Comparison of the fragmentation behavior of DNA and LNA single strands and duplexes.

TLDR
While DNA-DNA duplexes dissociated easily into single stranded fragments, the high stability of DNA-LNA hybrids resulted in predominant fragmentation of the DNA part rather than the LNA, while base losses were only observed from the DNA single strand of the hybrid.

DNA triple helices: biological consequences and therapeutic potential.

...

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