Nuclear transport of plant potyviral proteins.

  title={Nuclear transport of plant potyviral proteins.},
  author={M. Angela Restrepo and D D Freed and James C. Carrington},
  journal={The Plant cell},
  volume={2 10},
We have used immunoblotting, immunocytochemical, and gene fusion methods to examine the differential subcellular partitioning of tobacco etch potyvirus proteins that are potentially associated with RNA replication. From the earliest timepoints at which viral proteins could be detected, proteins Nla (49-kilodalton proteinase) and Nlb (58-kilodalton polymerase) were localized primarily in the nucleus, whereas the 71-kilodalton cylindrical inclusion protein was identified in the cytoplasm. The Nla… CONTINUE READING
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