Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells.

@article{Gmez2008NuclearTO,
  title={Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells.},
  author={Martha C. G{\'o}mez and Charles Earle Pope and Robert H Kutner and David M Ricks and L. A. Lyons and Mark T Ruhe and Cherie Dumas and Justine I Lyons and M Lopez and Betsy L Dresser and Jakob Reiser},
  journal={Cloning and stem cells},
  year={2008},
  volume={10 4},
  pages={469-83}
}
In the present study, we used the sand cat (Felis margarita) as a somatic cell donor to evaluate whether cryopreservation of donor cells alters viability and epigenetic events in donor cells and affects in vitro and in vivo developmental competence of derived embryos. In Experiment 1, flow cytometry analysis revealed that the percentage of necrosis and apoptosis in cells analyzed immediately after freezing/thawing (61 vs. 8.1%, respectively) was higher than that observed in frozen/thawed cells… CONTINUE READING
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