Nuclear transfer in cattle using in vivo-derived vs. in vitro-produced donor embryos: effect of developmental stage.

@article{Zakhartchenko1996NuclearTI,
  title={Nuclear transfer in cattle using in vivo-derived vs. in vitro-produced donor embryos: effect of developmental stage.},
  author={Valeri Zakhartchenko and Horst Dieter Reichenbach and Janet Riedl and Gustavo Adolfo Palma and Eckhard Wolf and Gottfried Brem},
  journal={Molecular reproduction and development},
  year={1996},
  volume={44 4},
  pages={493-8}
}
To determine the best developmental stage of donor embryos for yielding the highest number of clones per embryo, we compared the efficiencies of nuclear transfer when using blastomeres from morulae or morulae at cavitation, or when using inner-cell-mass cells of blastocysts as nuclear donors. This comparison was done both on in vivo-derived and in vitro-produced donor embryos. In experiment 1, with in vivo-derived donor embryos, nuclei from morulae at cavitation supported the development of… CONTINUE READING