• DOI:10.1152/AJPENDO.00098.2005
• Corpus ID: 8188673

Nuclear targeting of stanniocalcin to mammary gland alveolar cells during pregnancy and lactation.

@article{Hasilo2005NuclearTO,
  title={Nuclear targeting of stanniocalcin to mammary gland alveolar cells during pregnancy and lactation.},
  author={Craig P Hasilo and Christopher McCudden and J Ryan J Gillespie and Kathi A. James and Edward R Hirvi and Deenaz Zaidi and Graham Forsyth Wagner},
  journal={American journal of physiology. Endocrinology and metabolism},
  year={2005},
  volume={289 4},
  pages={
          E634-42
        }
}

• C. Hasilo, C. McCudden, +4 authors G. F. Wagner
• Published 1 October 2005
• Biology
• American journal of physiology. Endocrinology and metabolism

In most mammalian tissues, the stanniocalcin-1 gene (STC-1) produces a 50-kDa polypeptide hormone known as STC50. Within the ovaries, however, the STC-1 gene generates three higher-molecular-mass variants known as big STC. Big STC is targeted locally to corpus luteal cells to block progesterone release. During pregnancy and lactation, however, ovarian big STC production increases markedly, and the hormone is released into the serum. During lactation, this increase in hormone production is dependent on a suckling stimulus, suggesting that ovarian big STC may have regulatory effects on the lactating mammary gland. In this report, we have addressed this possibility. Our results revealed that virgin mammary tissue contained large numbers of membrane- and mitochondrial-associated STC receptors. However, as pregnancy progressed into lactation, there was a decline in receptor densities on both organelles and a corresponding rise in nuclear receptor density, most of which were on milk-producing, alveolar cells. This was accompanied by nuclear sequestration of the ligand. Sequestered STC resolved as one approximately 135-kDa band in the native state and therefore had the appearance of a big STC variant. However, chemical reduction collapsed this one band into six closely spaced, lower-molecular-mass species (28-41 kDa). Mammary gland STC production also underwent a dramatic shift during pregnancy and lactation. High levels of STC gene expression were observed in mammary tissue from virgin and pregnant rats. However, gene expression then fell to nearly undetectable levels during lactation, coinciding with the rise in nuclear targeting. These findings have thus shown that the mammary glands are indeed targeted by STC, even in the virgin state. They have further shown that there are marked changes in this targeting pathway during pregnancy and lactation, accompanied by a switch in ligand source (endogenous to exogenous). They also represent the first example of nuclear targeting by STC.