Nuclear targeting determinants of the phage P1 cre DNA recombinase.

@article{Le1999NuclearTD,
  title={Nuclear targeting determinants of the phage P1 cre DNA recombinase.},
  author={Yun Zheng Le and Sara Gagneten and Donatella Tombaccini and Birgit Bethke and Brian Sauer},
  journal={Nucleic acids research},
  year={1999},
  volume={27 24},
  pages={4703-9}
}
The Cre DNA recombinase of bacteriophage P1 has become a useful tool for genomic manipulation in mice and other eukaryotes. Because Cre is of prokaryotic origin, the 38 kDa protein has been presumed to gain access to the eukaryotic nucleus simply because it is sufficiently small to pass through the nuclear pore by passive diffusion. Instead, we show here that Cre carries nuclear targeting determinants that efficiently direct Cre entry into the nucleus of mammalian cells. Fusions of Cre with… CONTINUE READING

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