Nuclear substructure and dynamics

  title={Nuclear substructure and dynamics},
  author={A. Lamond and J. Sleeman},
  journal={Current Biology},
Many aspects of nuclear organization exhibit dynamic properties. Both nuclear bodies and individual chromatin regions can move through the nucleoplasm. Certain nuclear bodies are able to assemble and disassemble from soluble components in response to the metabolic demands of the cell. The cycling behaviour of nuclear factors and the rapid turnover of nuclear body components also underlines the importance of measuring the dynamic properties of proteins and RNPs when interpreting both… Expand
Physiological importance of RNA and protein mobility in the cell nucleus
  • T. Misteli
  • Biology, Medicine
  • Histochemistry and Cell Biology
  • 2007
The ability and mode of motion of proteins and RNAs has implications for how they find nuclear targets on chromatin and in nuclear subcompartments and how macromolecular complexes are assembled in vivo. Expand
Nuclear body movement is determined by chromatin accessibility and dynamics.
This finding suggests that the body mobility reflects the dynamics and accessibility of the chromatin environment, which might target bodies to specific nuclear subcompartments where they exert their biological function. Expand
Nucleolar proteome dynamics
The data establish a quantitative proteomic approach for the temporal characterization of protein flux through cellular organelles and demonstrate that the nucleolar proteome changes significantly over time in response to changes in cellular growth conditions. Expand
Mobility of multi-subunit complexes in the nucleus: accessibility and dynamics of chromatin subcompartments
Property of the chromatin environment can be identified from these experiments that determine the mobility of Cajal and PML bodies and other supramolecular complexes. Expand
Toward a High-Resolution View of Nuclear Dynamics
The impact of new technologies, especially in areas of fluorescence microscopy and proteomics, which are providing major insights into dynamic processes affecting both structure and function within the nucleus are reviewed. Expand
The intricacy of nuclear membrane dynamics during nucleophagy.
The process of nucleophagy is reviewed in the context of nuclear membrane dynamics, and the evidence for autophagic degradation of the nucleus in mammalian cells is examined. Expand
The intricacy of nuclear membrane dynamics during nucleophagy
The cell nucleus is an organelle bounded by a double-membrane which undergoes drastic reorganisation during major cellular events such as cell division and apoptosis. Maintenance of proper nuclearExpand
Labeling DNA Replication Foci to Visualize Chromosome Territories In Vivo
Experimental tools to follow chromatin dynamics by labeling DNA during S phase are described and a preservation of structural integrity is demonstrated which ensures that DNA from neighboring CTs is not able to mix freely within the same nuclear space. Expand
Spatial Organization of the Nucleus Compartmentalizes and Regulates the Genome
Findings that genome encoded regulatory elements such as enhancers can be hundreds of kb and even Mb apart further highlights the need for such a system as these distal elements must come together in the 3D space of the genome for their efficient functioning. Expand
Dynamics and interactions of parvoviral NS1 protein in the nucleus
Time‐lapse imaging of infected cells revealed that the intranuclear distribution of NS1‐EYFP evolves dramatically starting from the formation ofNS1 foci and proceeding to a homogenous distribution extending throughout the nucleus. Expand


Nuclear speckles: a model for nuclear organelles
Speckles are subnuclear structures that are enriched in pre-messenger RNA splicing factors and are located in the interchromatin regions of the nucleoplasm of mammalian cells, and can cycle continuously between speckles and other nuclear locations, including active transcription sites. Expand
Protein dynamics: implications for nuclear architecture and gene expression.
The observed dynamic properties of nuclear proteins are consistent with a central role for stochastic mechanisms in gene expression and nuclear architecture, and their dynamic interplay generates an ever-changing, but overall stable, architectural framework. Expand
The dynamics of chromosome organization and gene regulation.
  • D. Spector
  • Biology, Medicine
  • Annual review of biochemistry
  • 2003
The current state of knowledge with regard to the organization of chromosomes within the nucleus and the positioning of active versus inactive genes is described and studies on the dynamics of chromosomes and specific genetic loci within living cells and its relationship to gene activity and the cell cycle are discussed. Expand
The Contribution of Nuclear Compartmentalization to Gene Regulation
Current views on the origins of nuclear compartments and their roles in gene expression are discussed in this article. Expand
Cajal bodies and coilin—moving towards function
The Cajal body is an example of a conserved nuclear compartment that has been linked to molecular disease and offers clues about their function in the cell. Expand
Drosophila Neuroblasts Sequentially Express Transcription Factors which Specify the Temporal Identity of Their Neuronal Progeny
Drosophila neural precursors sequentially express the transcription factors Hunchback --> Krüppel --> Pdm --> Castor, with differentiated progeny maintaining the transcription factor profile present at their birth. Expand
The C elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable microRNA target.
It is shown that hbl-1, the C. elegans hunchback ortholog, also controls temporal patterning of the nervous system in Drosophila and is a probable target of microRNA regulation through its 3'UTR. Expand
Control of developmental timing in animals
  • A. Rougvie
  • Biology, Medicine
  • Nature Reviews Genetics
  • 2001
Key recent findings indicate that certain C. elegans timekeeping genes are conserved across phyla, and their developmental expression patterns indicate that a timing function might also be conserved. Expand
bantam Encodes a Developmentally Regulated microRNA that Controls Cell Proliferation and Regulates the Proapoptotic Gene hid in Drosophila
It is reported that the bantam gene of Drosophila encodes a 21 nucleotide microRNA that promotes tissue growth and identifies the pro-apoptotic gene hid as a target for regulation by bantam miRNA, providing an explanation for bantam's anti-APoptotic activity. Expand
microRNAs Tiny Regulators with Great Potential
Animal genomes contain an abundance of small genes that produce regulatory RNAs of about 22 nucleotides in length, and these microRNAs are diverse in sequence and expression patterns, suggesting that they may participate in a wide range of genetic regulatory pathways. Expand