Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors

  title={Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors},
  author={Stephen A. Adam and R S Marr and Larry Gerace},
  journal={The Journal of Cell Biology},
  pages={807 - 816}
We have developed an in vitro system involving digitonin-permeabilized vertebrate cells to study biochemical events in the transport of macromolecules across the nuclear envelope. While treatment of cultured cells with digitonin permeabilizes the plasma membranes to macromolecules, the nuclear envelopes remain structurally intact and nuclei retain the ability to transport and accumulate proteins containing the SV40 large T antigen nuclear location sequence. Transport requires addition of… CONTINUE READING
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