Nuclear membrane proteins with potential disease links found by subtractive proteomics.

@article{Schirmer2003NuclearMP,
  title={Nuclear membrane proteins with potential disease links found by subtractive proteomics.},
  author={Eric C Schirmer and Laurence Florens and Tinglu Guan and John R. Yates and Larry Gerace},
  journal={Science},
  year={2003},
  volume={301 5638},
  pages={
          1380-2
        }
}
To comprehensively identify integral membrane proteins of the nuclear envelope (NE), we prepared separately NEs and organelles known to cofractionate with them from liver. Proteins detected by multidimensional protein identification technology in the cofractionating organelles were subtracted from the NE data set. In addition to all 13 known NE integral proteins, 67 uncharacterized open reading frames with predicted membrane-spanning regions were identified. All of the eight proteins tested… CONTINUE READING
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