Nuclear magnetic resonance-based dissection of a glycosyltransferase specificity for the mucin MUC1 tandem repeat.

@article{Brokx2003NuclearMR,
  title={Nuclear magnetic resonance-based dissection of a glycosyltransferase specificity for the mucin MUC1 tandem repeat.},
  author={Richard D. Brokx and Leigh Revers and Qinghong Zhang and Shaoxian Yang and Tapas Kumar Mal and Mitsuhiko Ikura and Jean Gari{\'e}py},
  journal={Biochemistry},
  year={2003},
  volume={42 47},
  pages={13817-25}
}
The human glycoprotein MUC1 mucin plays a critical role in cancer progression. Breast, ovarian, and colon cancer cells often display unique cell-surface antigens corresponding to aberrantly glycosylated forms of the MUC1 tandem repeat. In this report, (15)N- and (13)C-labeled forms of a recombinant MUC1 construct containing five tandem repeats were used as substrates to define the order and kinetics of addition of N-acetylgalactosamine (GalNAc) moieties by a recombinant active form of the human… CONTINUE READING